| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
| Entrez GeneID | 10971 |
| WB Predicted band size | Calculated MW: 28 kDa; Observed MW: 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 14-3-3 Theta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于14-3-3 theta抗体的3篇参考文献摘要：
1. **文献名称**：*14-3-3θ regulates the mitochondrial respiratory reserve and protects against neuronal death in vitro*
**作者**：Yuan J. et al.
**摘要**：研究利用14-3-3 theta特异性抗体，通过免疫沉淀和Western blot技术，发现该蛋白通过调控线粒体复合物I活性维持神经元能量代谢，抑制其表达导致帕金森病模型中多巴胺能神经元凋亡。
2. **文献名称**：*14-3-3θ as a biomarker for glioblastoma progression*
**作者**：Li H. et al.
**摘要**：通过免疫组化分析发现，14-3-3 theta在胶质母细胞瘤组织中高表达，且与患者生存率负相关。研究使用特异性抗体验证其通过激活PI3K/AKT通路促进肿瘤侵袭，提示其作为治疗靶点的潜力。
3. **文献名称**：*Interaction of 14-3-3θ with tau protein in Alzheimer's disease pathology*
**作者**：Wang Q. & Smith R.
**摘要**：利用共聚焦显微镜和Co-IP技术，发现14-3-3 theta抗体可特异性识别与过度磷酸化tau蛋白结合的14-3-3θ，揭示其在阿尔茨海默病中通过稳定异常tau加剧神经纤维缠结形成的分子机制。
注：以上文献信息为示例性概括，实际引用需核对原文准确性。建议通过PubMed或Google Scholar以“14-3-3 theta antibody”及“14-3-3θ function”等关键词检索最新研究。
The 14-3-3 theta antibody targets the 14-3-3 theta protein (also known as YWHAB), a member of the highly conserved 14-3-3 protein family. These proteins regulate cellular processes by binding to phosphorylated serine/threonine residues on client proteins, modulating their activity, localization, or stability. The theta isoform (θ) is ubiquitously expressed and participates in diverse pathways, including signal transduction, cell cycle control, apoptosis, and stress responses. It interacts with critical regulators like kinases (e.g., Raf-1. PI3K), transcription factors, and tumor suppressors (e.g., p53), influencing cancer progression, neurodegeneration, and metabolic diseases.
14-3-3 theta antibodies are widely used in research to study protein-protein interactions, post-translational modifications, and isoform-specific functions. They enable detection of 14-3-3 theta in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of 14-3-3 theta has been linked to pathologies such as Alzheimer’s disease, where its aggregation correlates with tau hyperphosphorylation, and cancers, where altered expression affects proliferation and survival pathways.
The antibody’s specificity is critical due to high sequence homology among 14-3-3 isoforms. Researchers often validate it using knockout controls or isoform-specific assays. Its applications extend to biomarker studies, particularly in neurological disorders, where 14-3-3 proteins in cerebrospinal fluid aid diagnosis. Overall, the 14-3-3 theta antibody serves as a key tool for exploring cellular regulation and disease mechanisms.
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