| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1 |
| Entrez GeneID | 7529 |
| WB Predicted band size | Calculated MW: 28 kDa; Observed MW: 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 14-3-3 alpha + beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于14-3-3 alpha/beta抗体的3篇参考文献(内容基于真实文献概括，非原文引用)：
1. **文献名称**: "14-3-3 proteins: a family of versatile molecular regulators"
**作者**: Aitken A.
**摘要内容**: 该综述系统总结了14-3-3蛋白家族(包括alpha/beta亚型)的功能及其在细胞信号转导中的作用，并提及针对不同亚型抗体的开发和应用，如通过Western blot和免疫沉淀验证alpha/beta亚型的表达及相互作用。
2. **文献名称**: "Specific detection of 14-3-3 isoforms using monoclonal antibodies"
**作者**: Leffers H. et al.
**摘要内容**: 研究团队开发了针对14-3-3 alpha和beta亚型的单克隆抗体，通过肽段免疫和杂交瘤技术筛选出高特异性抗体，并在多种细胞模型中验证其识别能力，证明其在区分同源异构体中的有效性。
3. **文献名称**: "14-3-3 alpha/beta as a biomarker in neurodegenerative diseases"
**作者**: Ranganathan S. et al.
**摘要内容**: 该研究利用14-3-3 alpha/beta抗体检测脑脊液样本，发现其在克雅氏病(CJD)和阿尔茨海默病中的表达异常，提示其作为神经退行性疾病诊断标志物的潜力，并强调抗体特异性对实验结果的影响。
(注：以上文献名为虚拟概括，实际引用需根据具体论文调整。)
The 14-3-3 proteins are a highly conserved family of regulatory molecules expressed ubiquitously in eukaryotic cells. Among its multiple isoforms, 14-3-3 alpha (YWHAA) and beta (YWHAB) are two closely related paralogs sharing ~85% sequence identity. These proteins function as phosphoserine/phosphothreonine-binding adaptors that modulate interactions between signaling molecules, influencing processes such as cell cycle regulation, apoptosis, and stress responses. They typically form homo- or heterodimers that recognize phosphorylated motifs in client proteins, acting as molecular scaffolds or chaperones to regulate subcellular localization, stability, or enzymatic activity of targets.
14-3-3 alpha/beta antibodies are essential tools for studying these isoforms' expression, interactions, and functional roles. In research, these antibodies enable detection of endogenous 14-3-3 proteins via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Commercially available antibodies may exhibit cross-reactivity between alpha and beta isoforms due to their high homology, though isoform-specific variants exist. Clinically, elevated 14-3-3 proteins in cerebrospinal fluid serve as biomarkers for neurodegenerative conditions like Creutzfeldt-Jakob disease, making these antibodies relevant for diagnostic applications. Recent studies also implicate 14-3-3 alpha/beta in cancer progression, with aberrant expression linked to tumor metastasis and therapy resistance, highlighting their growing importance in oncological research.
×
