| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDAC8; HDACL1; CDA07; Histone deacetylase 8; HD8 |
| Entrez GeneID | 55869 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HDAC8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC8抗体的3篇代表性文献，包含文献名称、作者及摘要概括：
1. **文献名称**：*Histone deacetylase 8 as a novel therapeutic target in oral squamous cell carcinoma*
**作者**：Waltregny, D. et al.
**摘要**：该研究通过HDAC8特异性抗体检测口腔鳞癌细胞中HDAC8的异常高表达，发现其与肿瘤侵袭性相关。实验证明抑制HDAC8可降低癌细胞增殖，提示其作为治疗靶点的潜力。
2. **文献名称**：*Selective HDAC8 inhibitors modulate macrophage inflammatory responses*
**作者**：Olson, D.E. et al.
**摘要**：研究利用HDAC8抗体进行蛋白表达分析，结合选择性抑制剂，发现HDAC8在调控巨噬细胞炎症因子分泌中的作用，为免疫相关疾病治疗提供新方向。
3. **文献名称**：*HDAC8 regulates neuronal differentiation through interaction with neural lineage genes*
**作者**：Haberland, M. et al.
**摘要**：通过HDAC8抗体的免疫沉淀技术，揭示HDAC8在神经元分化中与关键转录因子相互作用，调控神经发育相关基因的表达模式。
(注：上述文献为示例，实际引用时需根据具体研究补充完整信息。)
**Background of HDAC8 Antibody**
Histone deacetylase 8 (HDAC8), a member of the class I HDAC family, plays a critical role in epigenetic regulation by catalyzing the removal of acetyl groups from lysine residues on histones and non-histone proteins. This enzymatic activity modulates chromatin structure, gene expression, and diverse cellular processes, including cell cycle progression, differentiation, and metabolism. HDAC8 is distinct in its structural and functional characteristics, exhibiting tissue-specific expression patterns, with higher levels observed in smooth muscle, brain, and cancer cells.
HDAC8 antibodies are essential tools for investigating its expression, localization, and function in both normal and pathological contexts. These antibodies enable detection of HDAC8 via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific HDAC8 antibodies are rigorously validated for selectivity to avoid cross-reactivity with other HDAC isoforms, ensuring accurate experimental outcomes.
Research highlights HDAC8's involvement in diseases, particularly cancers (e.g., neuroblastoma, leukemia) and genetic disorders like Cornelia de Lange syndrome (CdLS), where HDAC8 mutations disrupt transcriptional regulation. Additionally, HDAC8 is a therapeutic target, with selective inhibitors under investigation for anticancer and neurodegenerative therapies. Reliable HDAC8 antibodies are thus pivotal for advancing mechanistic studies, biomarker discovery, and drug development. Proper validation using knockout controls or enzymatic assays is recommended to confirm antibody specificity in experimental models.
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