| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1 |
| Entrez GeneID | 4303 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FoxO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于FOXO4抗体的3篇参考文献示例(注：文献为模拟示例，实际引用需核实原文)：
---
1. **标题**: "FOXO4-dependent regulation of cellular senescence and oxidative stress"
**作者**: Wang B, et al.
**摘要**: 本研究利用FOXO4特异性抗体通过免疫印迹和免疫荧光技术，揭示了FOXO4在调控衰老细胞存活中的作用。研究发现，FOXO4通过与p53相互作用维持衰老细胞稳态，其抗体在分离蛋白复合物中表现出高特异性。
---
2. **标题**: "FOXO4 expression in prostate cancer: Immunohistochemical analysis using a novel monoclonal antibody"
**作者**: Smith JL, et al.
**摘要**: 开发了一种新型FOXO4单克隆抗体，并通过免疫组化验证其在前列腺癌组织中的表达。结果显示FOXO4低表达与肿瘤侵袭性相关，抗体在区分正常与癌变组织中具有高灵敏度和特异性。
---
3. **标题**: "FOXO4 modulates autophagy via antibody-validated transcriptional targets in diabetic nephropathy"
**作者**: Chen H, et al.
**摘要**: 通过FOXO4抗体进行染色质免疫沉淀(ChIP)分析，发现FOXO4直接调控自噬相关基因(如LC3)。研究证实FOXO4在糖尿病肾病中通过影响自噬通路加剧肾损伤，抗体在ChIP实验中表现稳定。
---
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“FOXO4 antibody”、“FOXO4 immunohistochemistry”等获取最新研究。
FOXO4 antibodies are essential tools for studying the Forkhead box O4 (FOXO4) protein, a member of the FOXO family of transcription factors. FOXO4 plays a critical role in regulating cellular processes such as cell cycle arrest, oxidative stress response, DNA repair, and apoptosis. It functions by translocating to the nucleus under stress conditions, where it activates or represses target genes involved in tumor suppression, longevity, and metabolic regulation. Dysregulation of FOXO4 has been implicated in cancer, aging-related diseases, and metabolic disorders, making it a key focus in biomedical research.
FOXO4 antibodies are typically monoclonal or polyclonal and are designed to detect specific epitopes within the protein’s conserved forkhead DNA-binding domain (DBD) or regulatory regions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to analyze FOXO4 expression, localization, and interactions. Researchers often validate antibody specificity using knockout (KO) cell lines or siRNA-mediated FOXO4 knockdown.
Recent studies highlight FOXO4's role in cellular senescence, particularly its interaction with p53 to promote senescent cell survival. Inhibiting FOXO4-DBD interactions has emerged as a strategy to target senescence-associated secretory phenotype (SASP) in aging tissues. FOXO4 antibodies thus support investigations into aging mechanisms, cancer therapy resistance, and metabolic diseases. Commercial FOXO4 antibodies are available from suppliers like Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, with validation data provided for reliability across experimental models.
×
