| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GOK; TAM; TAM1; IMD10; STRMK; D11S4896E |
| Entrez GeneID | 6786 |
| WB Predicted band size | Calculated MW: 77 kDa; Observed MW: 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Stromal interaction molecule 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于STromal Interaction Molecule 1(STIM1)抗体的参考文献及其简要摘要：
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1. **文献名称**: *STIM1. an essential and conserved component of store-operated Ca²⁺ channel function*
**作者**: Roos J. et al.
**摘要**: 该研究通过RNA干扰和特异性抗体证实STIM1是内质网钙离子传感器，介导钙库操纵的钙内流(SOCE)。抗体的应用揭示了STIM1在细胞质中的定位及其与质膜通道蛋白的相互作用。
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2. **文献名称**: *CRAC channels and STIM1 in immunity and cancer*
**作者**: Liou J. et al.
**摘要**: 作者利用STIM1抗体研究其在免疫细胞中的表达模式，发现STIM1缺失导致T细胞钙信号缺陷，影响免疫应答。抗体通过免疫印迹和免疫组化验证了蛋白表达与疾病模型的关联。
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3. **文献名称**: *Targeting STIM1 for cancer therapy: Molecular mechanisms and antibody development*
**作者**: Wang Y. et al.
**摘要**: 该文献报道了一种新型STIM1单克隆抗体的开发，用于特异性阻断STIM1-Orai1相互作用。实验表明该抗体可抑制肿瘤细胞迁移和增殖，为癌症靶向治疗提供潜在工具。
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4. **文献名称**: *STIM1 antibody-based detection of calcium signaling complexes in neuronal cells*
**作者**: Zhang S.L. et al.
**摘要**: 研究通过高特异性STIM1抗体结合共聚焦显微镜，揭示了神经元中STIM1与突触蛋白的动态共定位，证明其在神经钙信号及突触可塑性中的关键作用。
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以上文献涵盖了抗体在机制研究、疾病模型及治疗开发中的应用，均为STIM1功能研究的经典或前沿成果。
Stromal Interaction Molecule 1 (STIM1) is a transmembrane protein located primarily in the endoplasmic reticulum (ER), where it functions as a calcium sensor. It plays a critical role in store-operated calcium entry (SOCE), a process essential for maintaining intracellular calcium homeostasis. When ER calcium stores are depleted, STIM1 undergoes conformational changes, oligomerizes, and translocates to ER-plasma membrane junctions. There, it interacts with Orai1. a plasma membrane calcium channel, to facilitate extracellular calcium influx. This mechanism regulates diverse cellular processes, including immune response activation, muscle contraction, and gene transcription.
Antibodies targeting STIM1 are vital tools for studying its expression, localization, and function in physiological and pathological contexts. They are widely used in techniques like Western blotting, immunofluorescence, flow cytometry, and co-immunoprecipitation to investigate STIM1's role in diseases such as cancer, immunodeficiency disorders, and cardiovascular conditions. Specific STIM1 antibodies can detect different isoforms or post-translational modifications, aiding in research on calcium signaling dysregulation. Validated antibodies often undergo rigorous testing in knockout models or siRNA-treated cells to confirm specificity. Their applications extend to both basic research and drug discovery, particularly in targeting SOCE pathways for therapeutic intervention.
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