| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAB7; PRO2706 |
| Entrez GeneID | 7879 |
| WB Predicted band size | Calculated MW: 23 kDa; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RAB7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Rab7抗体的3篇参考文献的简要信息：
1. **文献名称**: "Role of Rab7 in the regulation of lysosomal functions"
**作者**: Vanlandingham PA, Ceresa BP
**摘要**: 该研究使用Rab7特异性抗体进行Western blot和免疫荧光，揭示Rab7在内体-溶酶体运输中的关键作用，并探讨其与溶酶体酶活性和自噬过程的关联。
2. **文献名称**: "Rab7 modulates T cell receptor signaling by altering lysosomal function"
**作者**: Schmid SL, Damke H
**摘要**: 通过Rab7抗体阻断实验，文章发现Rab7通过调控溶酶体成熟影响T细胞受体信号转导，为免疫调节提供了新机制。
3. **文献名称**: "Rab7 is required for the cellular entry of SARS-CoV-2"
**作者**: Wang H, et al.
**摘要**: 研究利用Rab7抗体进行共定位分析，证实Rab7在新冠病毒内吞过程中的必要性，为抗病毒治疗靶点开发提供依据。
(注：以上文献信息为示例性内容，实际引用时需核对真实文献数据及数据库来源。)
Rab7 is a small GTPase belonging to the Rab family, which plays a critical role in regulating membrane trafficking pathways, particularly in the late endosomal and lysosomal compartments. It governs the maturation of late endosomes, their fusion with lysosomes, and the retrograde transport of cargo to the trans-Golgi network. Rab7 is also involved in autophagy, mediating autophagosome-lysosome fusion, and in pathogen clearance through phagosome maturation. Dysregulation of Rab7 has been linked to various diseases, including neurodegenerative disorders (e.g., Alzheimer’s and Charcot-Marie-Tooth disease), cancer metastasis, and infectious diseases caused by intracellular pathogens.
Rab7 antibodies are essential tools for studying these processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect Rab7 expression levels, subcellular localization, and interactions with effector proteins. Polyclonal and monoclonal antibodies targeting Rab7 are typically raised against conserved regions of the protein (e.g., human/mouse residues 1-207) and validated for specificity using knockout cell lines or siRNA knockdown. Some antibodies distinguish between active (GTP-bound) and inactive (GDP-bound) Rab7 conformations, aiding functional studies. Species cross-reactivity (e.g., human, mouse, rat) is a key consideration for experimental design. Rab7 antibodies have advanced research in intracellular trafficking mechanisms, disease pathology, and therapeutic targeting of Rab7-associated pathways.
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