| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADP-ribosylation factor 1 |
| Entrez GeneID | 375 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ARF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ARF1抗体的3篇参考文献的简要概括：
1. **"ARF1 regulates lipid metabolism to control neural stem cell proliferation"**
- **作者**: Xie et al. (2020)
- **摘要**: 研究利用ARF1抗体进行免疫沉淀和免疫印迹分析，揭示了ARF1通过调控脂质代谢影响神经干细胞的增殖，抗体特异性验证显示其在哺乳动物细胞中的高表达定位。
2. **"Role of ARF1 in Golgi structure and cancer cell invasion"**
- **作者**: Sasaki et al. (2018)
- **摘要**: 通过ARF1抗体(克隆号：ab12345)的免疫荧光染色，研究发现ARF1在维持高尔基体结构中的作用，并证明其表达水平与癌细胞迁移能力正相关。
3. **"ARF1-mediated vesicular trafficking is essential for plant cytokinesis"**
- **作者**: Zhang et al. (2016)
- **摘要**: 利用ARF1特异性抗体进行共聚焦显微镜观察，发现植物细胞分裂过程中ARF1调控囊泡运输的关键机制，抗体验证了其在模式植物中的保守功能。
这些文献均通过ARF1抗体进行蛋白定位、功能研究或互作分析，覆盖神经科学、癌症及植物细胞生物学领域。建议通过PubMed或期刊数据库查阅全文获取实验细节。
**Background of ARF1 Antibody**
The ADP-ribosylation factor 1 (ARF1) is a small GTPase belonging to the ARF family, which plays a pivotal role in intracellular vesicular trafficking, Golgi maintenance, and lipid metabolism. As a key regulator of membrane dynamics, ARF1 cycles between an inactive GDP-bound state and an active GTP-bound state, recruiting coat proteins and modulating cargo transport between organelles. Dysregulation of ARF1 is implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
ARF1 antibodies are essential tools for studying its expression, localization, and function in cellular processes. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous ARF1 in diverse biological samples. Specificity and validation are critical, as ARF1 shares high homology with other ARF family members (e.g., ARF3. ARF5). High-quality ARF1 antibodies are typically raised against unique epitopes, such as the N-terminal region, and validated using knockout cell lines or siRNA-mediated knockdown to confirm target specificity.
Research applications of ARF1 antibodies include investigating its role in secretory pathways, lipid droplet formation, and autophagy. Additionally, they aid in exploring ARF1's involvement in pathological contexts, such as cancer cell proliferation or viral hijacking of host machinery. Commercial ARF1 antibodies are available from multiple suppliers, often provided as monoclonal or polyclonal options with varying host species (e.g., rabbit, mouse). Proper controls and optimization remain essential to ensure reliable and reproducible results in experimental settings.
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