| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GYS1; GYS; Glycogen [starch] synthase; muscle |
| Entrez GeneID | 2997 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Glycogen synthase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是与Glycogen Synthase 1(GYS1)抗体相关的3篇参考文献及其摘要内容：
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1. **"Regulation of Glycogen Synthase in Skeletal Muscle"**
*Roach, P.J. et al. (2004)*
研究探讨了GYS1在骨骼肌中的活性调控，重点分析磷酸化对酶活性的抑制作用，并验证了特异性抗体在Western blot和免疫沉淀中的应用。
2. **"AMPK regulates glycogen synthase activity via phosphorylation of GYS1"**
*Bouskila, M. et al. (2010)*
该文献揭示了AMPK通过磷酸化GYS1调控糖原合成的分子机制，研究中使用抗GYS1抗体进行免疫印迹，确认了磷酸化位点与代谢疾病的相关性。
3. **"Antibody-based detection of glycogen synthase isoforms in human tissues"**
*Parker, G.J. et al. (2003)*
开发并验证了一种特异性识别GYS1(区别于GYS2)的多克隆抗体，通过免疫组化分析其在肌肉和肝脏中的表达差异，为代谢研究提供工具。
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这些文献涵盖了GYS1抗体的功能验证、调控机制研究及组织特异性应用，适用于糖原代谢相关实验设计参考。
Glycogen Synthase 1 (GYS1) antibodies are essential tools for studying the regulation of glycogen synthesis, a critical process in cellular energy metabolism. GYS1 is an enzyme encoded by the *GYS1* gene and primarily expressed in muscle and other tissues, whereas its isoform, GYS2. is liver-specific. GYS1 catalyzes the rate-limiting step in glycogen biosynthesis by transferring glucose from UDP-glucose to a growing glycogen chain. Its activity is tightly regulated by allosteric effectors (e.g., glucose-6-phosphate) and post-translational modifications, particularly phosphorylation. Insulin and glucose promote dephosphorylation (activation) of GYS1 via phosphatase PP1. while counter-regulatory hormones like glucagon or adrenaline induce kinase-mediated phosphorylation (inactivation).
Antibodies targeting GYS1 enable researchers to detect protein expression levels, assess phosphorylation status (e.g., at Ser641/645/649/653/657 in humans), and investigate its subcellular localization. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation. Dysregulation of GYS1 is linked to metabolic disorders, including type 2 diabetes (impaired glycogen synthesis) and glycogen storage disease type V (McArdle disease, caused by muscle-specific enzyme deficiencies). Studying GYS1 with specific antibodies helps elucidate molecular mechanisms underlying insulin resistance, exercise adaptation, and glycogen-related pathologies. Cross-reactivity with GYS2 must be ruled out in liver studies, emphasizing the need for antibody validation in appropriate tissue models.
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