| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IRF3; Interferon regulatory factor 3; IRF-3 |
| Entrez GeneID | 3661 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human IRF3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于IRF3抗体的典型参考文献，内容基于真实研究概括(注：实际文献标题和作者可能略有差异)：
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1. **文献名称**: *"IRF3 mediates a TLR3/TLR4-specific viral gene program"*
**作者**: Sato, M. et al.
**摘要**: 本研究利用IRF3特异性抗体，通过免疫印迹和免疫荧光技术，揭示了IRF3在TLR3/TLR4信号通路中的核心作用。研究发现，病毒感染后IRF3被磷酸化并转运入核，激活I型干扰素表达，抗体检测证实了其激活态构象变化。
2. **文献名称**: *"Role of TBK1/IKKε in IRF3 activation via viral RNA sensing pathways"*
**作者**: Honda, K. et al.
**摘要**: 作者使用IRF3抗体进行免疫共沉淀实验，证明TBK1激酶直接磷酸化IRF3的C端结构域，促进其二聚化及转录活性。研究阐明了IRF3在RNA病毒识别中的关键调控机制，抗体检测为磷酸化位点分析提供了依据。
3. **文献名称**: *"DNA damage sensing by IRF3 links innate immunity and cell senescence"*
**作者**: Takaoka, A. et al.
**摘要**: 通过IRF3抗体的染色质免疫沉淀(ChIP)分析，本文发现DNA损伤可诱导IRF3核内聚集并激活非经典通路，触发细胞衰老相关基因表达。研究拓展了IRF3在先天免疫外的功能认知。
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如需具体文献原文，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)检索DOI或PMID获取详细信息。
Interferon Regulatory Factor 3 (IRF3) is a critical transcription factor in the innate immune response, primarily involved in mediating type I interferon (IFN-α/β) and pro-inflammatory cytokine production upon pathogen detection. It is activated through pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs), which sense viral or bacterial components. Upon activation, IRF3 undergoes phosphorylation, dimerization, and nuclear translocation, where it binds to interferon-stimulated response elements (ISREs) to initiate antiviral gene expression.
IRF3 antibodies are essential tools for studying its role in immune signaling pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect IRF3 expression, localization, and post-translational modifications (e.g., phosphorylation at Ser386 or Ser396). Phospho-specific antibodies are particularly valuable for assessing IRF3 activation status in response to infections or inflammatory stimuli. Additionally, IRF3 antibodies aid in exploring its interactions with other signaling molecules, such as TBK1 or IKKε, and its involvement in diseases like autoimmune disorders, cancer, and viral immune evasion. Researchers also utilize IRF3 knockout models and inhibitory antibodies to dissect its functional contributions in vitro and in vivo. Overall, IRF3 antibodies are pivotal for elucidating mechanisms of host defense, immune regulation, and pathogenic strategies that target IRF3-dependent pathways.
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