| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MT-CO2; COX2; CO2; Cytochrome c oxidase II; MTCO2; COII; COXII |
| Entrez GeneID | 4513 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human MTCO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MTCO2抗体的3篇参考文献的简要概括(文献信息为虚构示例，仅供格式参考)：
1. **《MTCO2 as a mitochondrial marker in neurodegenerative diseases》**
*作者：Smith A, et al.*
摘要：该研究利用MTCO2抗体检测阿尔茨海默病模型小鼠脑组织中线粒体复合物IV的亚基表达水平，发现MTCO2蛋白表达显著降低，提示其可能作为神经退行性疾病的潜在生物标志物。
2. **《Validation of a novel monoclonal antibody for MTCO2 in human colorectal cancer》**
*作者：Chen L, et al.*
摘要：研究报道了一种新型MTCO2单克隆抗体的开发，并通过免疫组化验证其在结直肠癌组织中的特异性结合，结果显示MTCO2表达与肿瘤代谢异常及患者预后相关。
3. **《MTCO2 antibody-based profiling of mitochondrial dysfunction in skeletal muscle》**
*作者：Garcia R, et al.*
摘要：通过MTCO2抗体结合Western blot和免疫荧光技术，分析了线粒体肌病患者骨骼肌中线粒体呼吸链功能异常，证实MTCO2蛋白缺失与疾病严重程度呈正相关。
The MTCO2 antibody is a crucial tool in mitochondrial research, targeting the Mitochondrial Cytochrome C Oxidase Subunit II (MTCO2), a key component of Complex IV in the electron transport chain (ETC). MTCO2 is encoded by mitochondrial DNA and plays a central role in oxidative phosphorylation, facilitating the transfer of electrons from cytochrome c to molecular oxygen, a critical step in ATP production. This subunit is integral to maintaining cellular energy homeostasis and is often studied in contexts of mitochondrial dysfunction, metabolic disorders, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and cancer.
The MTCO2 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and mitochondrial integrity. Its specificity helps identify defects in mitochondrial structure or function, such as in mitochondrial encephalomyopathies or cancers with altered metabolic profiles. Researchers also employ this antibody to explore cellular responses to oxidative stress, metabolic adaptations, and therapeutic interventions targeting mitochondrial pathways.
Commercial MTCO2 antibodies are typically validated across species (human, mouse, rat) and require verification of mitochondrial specificity via controls like knockout cells or mitochondrial isolation protocols. Proper application aids in elucidating disease mechanisms linked to ETC disruptions and evaluating potential biomarkers or therapeutic targets in mitochondrial-associated pathologies.
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