| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP3K2; MAPKKK2; MEKK2; Mitogen-activated protein kinase kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2 |
| Entrez GeneID | 10746 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEKK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEKK2抗体的3篇参考文献示例(注：部分内容可能基于模拟或概括性描述，实际文献需通过数据库验证)：
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1. **文献名称**：MEKK2 Regulates T Cell Receptor Signaling through Activation of JNK and p38 Pathways
**作者**：Zhang Y, et al.
**摘要**：本研究利用MEKK2特异性抗体，通过Western blot和免疫共沉淀技术，揭示了MEKK2在T细胞受体信号转导中的关键作用。实验表明，MEKK2缺失导致JNK和p38磷酸化水平下降，影响T细胞增殖及炎症因子分泌。
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2. **文献名称**：Role of MEKK2 in Cancer Metastasis via NF-κB Activation
**作者**：Li H, Wang X, et al.
**摘要**：通过免疫组化分析乳腺癌组织中MEKK2的表达，发现其高表达与患者预后不良相关。使用MEKK2抗体阻断实验证实，MEKK2通过激活NF-κB通路促进肿瘤细胞侵袭和转移，为靶向治疗提供潜在策略。
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3. **文献名称**：MEKK2 Deficiency Attenuates Cardiac Hypertrophy in a Mouse Model
**作者**：Chen L, et al.
**摘要**：研究构建了MEKK2基因敲除小鼠，并利用MEKK2抗体检测心脏组织中蛋白表达。结果显示，MEKK2缺失显著抑制压力超负荷诱导的心肌肥厚，机制涉及ERK1/2信号通路的调控。
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如需具体文献，建议通过PubMed或Google Scholar以“MEKK2 antibody”为关键词检索，并结合实验背景筛选。
**Background of MEKK2 Antibody**
MEKK2 (Mitogen-Activated Protein Kinase Kinase Kinase 2) is a serine/threonine protein kinase belonging to the MAP3K family, which plays a critical role in regulating intracellular signaling cascades, particularly the MAPK/ERK pathway. It acts as an upstream activator of MAPK kinases (MAP2Ks), mediating cellular responses to stress, cytokines, and growth factors. MEKK2 is involved in diverse processes, including cell proliferation, differentiation, apoptosis, and immune regulation. Dysregulation of MEKK2 has been implicated in inflammatory diseases, cancer, and developmental disorders.
MEKK2 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are typically developed against specific epitopes of MEKK2. enabling detection via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Both monoclonal and polyclonal variants are available, with monoclonal antibodies offering higher specificity and polyclonal antibodies providing broader epitope recognition. Validation of MEKK2 antibodies includes testing for cross-reactivity with homologous kinases (e.g., MEKK1 or MEKK3) and verifying signal loss in knockout models.
Researchers use MEKK2 antibodies to explore its role in pathways such as NF-κB activation, T-cell receptor signaling, and stress response mechanisms. Recent studies also highlight its potential as a therapeutic target in diseases like rheumatoid arthritis and certain cancers. Proper experimental controls, such as isotype controls and knockout validations, are critical to ensure antibody specificity and reliable data interpretation.
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