| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Entrez GeneID | 5925 |
| WB Predicted band size | Calculated MW: 106 kDa; Observed MW: 106 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Retinoblastoma |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Rb抗体的参考文献示例(内容为假设性示例，仅供格式参考)：
1. **文献名称**：*"Monoclonal Antibodies Against Retinoblastoma Protein for Tumor Diagnosis"*
**作者**：Smith J et al.
**摘要**：该研究开发了针对Rb蛋白的单克隆抗体，验证了其在免疫组化中检测肿瘤样本中Rb表达缺失的应用，显示与多种癌症的临床分期相关。
2. **文献名称**：*"Rb Phosphorylation Status Regulates Cell Cycle Progression: Insights from Antibody-Based Assays"*
**作者**：Lee C, Weinberg RA
**摘要**：通过磷酸化特异性Rb抗体，揭示了Rb蛋白不同磷酸化状态在细胞周期调控中的动态变化，为癌症治疗靶点提供了依据。
3. **文献名称**：*"Therapeutic Targeting of Rb Pathway via Antibody-Mediated Delivery"*
**作者**：Zhang H et al.
**摘要**：利用Rb抗体偶联药物靶向递送治疗分子，在动物模型中成功抑制了Rb缺陷型肿瘤的生长，展示了抗体在精准治疗中的潜力。
4. **文献名称**：*"Comparative Analysis of Commercial Rb Antibodies in Western Blotting"*
**作者**：Brown K et al.
**摘要**：评估了市售多种Rb抗体的特异性与灵敏度，为实验中选择合适抗体提供了数据支持，强调不同表位设计对结果的影响。
(注：以上内容为模拟示例，实际文献需通过学术数据库检索获取。)
**Background of Rb Antibody**
The retinoblastoma (Rb) protein, encoded by the *RB1* gene, is a critical tumor suppressor regulating cell cycle progression. Discovered through its association with retinoblastoma, a pediatric eye cancer, Rb inhibits uncontrolled cell division by binding to E2F transcription factors, blocking S-phase entry. Its activity is modulated by phosphorylation; hypophosphorylated Rb actively suppresses cell cycle progression, while hyperphosphorylation inactivates it, allowing cell division. Dysfunctional Rb, due to mutations or pathway disruptions (e.g., cyclin-CDK overactivation), is implicated in various cancers.
Rb antibodies are essential tools in research and diagnostics, detecting Rb expression, phosphorylation status, or interactions in techniques like Western blotting, immunohistochemistry, or co-immunoprecipitation. They help study Rb's role in cancer biology, apoptosis, and differentiation. Clinically, Rb antibody-based assays assess tumor suppressor status in cancers (e.g., breast, lung, prostate) or evaluate retinoblastoma pathogenesis. Recent studies also explore Rb's involvement in senescence, metabolic regulation, and response to targeted therapies, underscoring its multifaceted role beyond cell cycle control. These antibodies remain pivotal in advancing oncology research and precision medicine.
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