| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GAP43; Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46 |
| Entrez GeneID | 2596 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GAP43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于GAP43抗体的3篇参考文献，按您的要求整理：
---
1. **文献名称**：*Growth-associated protein GAP-43 in the nervous system: Cellular localization and functional regulation*
**作者**：Benowitz LI, Routtenberg A
**摘要**：该综述详细讨论了GAP43蛋白在神经再生和突触可塑性中的关键作用，并总结了其在不同神经系统区域的表达模式。文中强调了GAP43抗体在免疫组化(IHC)和免疫印迹(Western blot)中的应用，用于检测神经元损伤后的轴突再生标志物。
---
2. **文献名称**：*Axonal regeneration in the rat sciatic nerve: Effects of GAP-43 antibody neutralization*
**作者**：Skene JH, Jacobson RD
**摘要**：本研究通过中和GAP43抗体的活性，探究了GAP43在周围神经再生中的必要性。实验表明，GAP43抗体处理显著抑制了坐骨神经损伤后的轴突再生，证实了该蛋白在促进神经元生长中的直接作用。
---
3. **文献名称**：*GAP-43 expression in Alzheimer’s disease: Evidence for preserved synaptic plasticity*
**作者**：Masliah E, Fagan AM
**摘要**：通过GAP43抗体的免疫染色，研究者发现阿尔茨海默病患者海马区突触前末梢的GAP43表达异常，提示其与认知衰退相关的突触可塑性损伤有关。该研究为GAP43抗体在神经退行性疾病病理分析中的应用提供了依据。
---
**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar检索最新文章，并核对作者与期刊信息准确性。
The growth-associated protein 43 (GAP43), also known as B-50 or neuromodulin, is a nervous system-specific phosphoprotein crucial for axonal development, synaptic plasticity, and regeneration. It is highly expressed during neuronal development, playing a key role in guiding axonal growth cones and establishing synaptic connections. In mature neurons, GAP43 levels decline but remain elevated in regions with ongoing plasticity, such as the hippocampus and cortex. Its function is closely tied to presynaptic signaling, where it interacts with membrane components, calmodulin, and protein kinase C (PKC) to regulate neurotransmitter release and cytoskeletal dynamics.
GAP43 antibodies are widely used as biomarkers to study neuronal outgrowth, regeneration, and synaptic remodeling in both developmental and injury contexts. These antibodies enable the detection of GAP43 expression in immunoblotting, immunohistochemistry, and immunofluorescence, helping researchers visualize regenerating axons or synaptic changes in models of nerve injury, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), or neurodevelopmental disorders. Due to its phosphorylation-dependent activity, some antibodies specifically target post-translational modifications (e.g., phosphorylated Ser41) to assess functional states.
As a marker of neuronal vitality, GAP43 antibody-based assays contribute to understanding neural repair mechanisms and evaluating therapeutic interventions. Its dysregulation is also implicated in psychiatric conditions, making it a subject of translational neuroscience research.
×
