| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP |
| Entrez GeneID | 3190 |
| WB Predicted band size | Calculated MW: 51 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human hnRNP K |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于hnRNP K抗体的参考文献信息(基于公开文献概括，非真实引用，仅供参考)：
1. **文献名称**: "hnRNP K interacts with RNA polymerase II and regulates c-Myc transcription"
**作者**: Michelotti EF 等
**摘要**: 研究利用hnRNP K抗体通过ChIP实验证明hnRNP K直接结合c-Myc基因启动子区，调控其转录活性，提示其在细胞增殖中的作用。
2. **文献名称**: "hnRNP K overexpression alters RNA splicing and promotes leukemogenesis"
**作者**: Zhou Z 等
**摘要**: 通过Western blot和免疫组化(使用hnRNP K特异性抗体)，发现hnRNP K在急性髓系白血病中高表达，并通过异常剪接驱动肿瘤发生。
3. **文献名称**: "Proteomic analysis of hnRNP K-associated complexes in DNA damage response"
**作者**: Moumen A 等
**摘要**: 使用hnRNP K抗体进行免疫共沉淀，鉴定了其在DNA损伤修复中与ATM/ATR激酶的相互作用，揭示其维持基因组稳定的新功能。
4. **文献名称**: "Differential expression of hnRNP K in prostate cancer correlates with disease progression"
**作者**: Barboro P 等
**摘要**: 通过免疫组织化学(抗hnRNP K抗体)发现hnRNP K在转移性前列腺癌中显著上调，可作为预后标志物及潜在治疗靶点。
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注：以上文献信息为示例性质，实际引用需根据具体研究查找真实文献。
Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional RNA/DNA-binding protein involved in regulating gene expression at transcriptional and post-transcriptional levels. It belongs to the hnRNP family, which plays critical roles in mRNA processing, transport, and stability. hnRNP K contains three K-homology (KH) domains that mediate interactions with nucleic acids, particularly poly(C)-rich sequences. It participates in diverse cellular processes, including chromatin remodeling, transcription, translation, and signal transduction, often acting as a scaffold to integrate signaling pathways with nucleic acid metabolism.
Antibodies targeting hnRNP K are essential tools for studying its localization, interactions, and functional roles. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate hnRNP K's involvement in cellular responses to stress, DNA damage, and oncogenic transformation. These antibodies help characterize its shuttling between nuclear and cytoplasmic compartments, as well as its association with specific mRNAs or regulatory proteins. Many studies employ hnRNP K antibodies to explore its implication in diseases, particularly cancer, where its expression levels often correlate with tumor progression and patient prognosis. Commercially available antibodies are typically raised against conserved regions of the protein, with validation including knockout cell line controls or siRNA-mediated knockdown. Proper validation remains crucial due to potential cross-reactivity with other hnRNP family members.
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