| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCNE2; G1/S-specific cyclin-E2 |
| Entrez GeneID | 9134 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cyclin E2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Cyclin E2抗体的文献参考，包含名称、作者及摘要概括：
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1. **文献名称**：*Cyclin E2. a novel human G1 cyclin and interacting partner of the p27Kip1 protein*
**作者**：Lauper, N. et al. (1998)
**摘要**：该研究首次克隆并鉴定了Cyclin E2基因，通过抗体验证其在细胞周期G1/S转换中的作用，发现其与p27Kip1蛋白的相互作用，提示其在细胞增殖调控中的关键功能。
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2. **文献名称**：*Overexpression of cyclin E2 is associated with tumor progression in clear cell renal cell carcinoma*
**作者**：Shi, Y. et al. (2019)
**摘要**：研究利用Cyclin E2抗体进行免疫组化分析，发现Cyclin E2在肾透明细胞癌中高表达，且与肿瘤分期、转移及患者生存率显著相关，提示其作为潜在预后标志物的价值。
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3. **文献名称**：*Cyclin E2 regulates the compensatory expansion of β-cells in response to insulin resistance*
**作者**：Fiaschi-Taesch, N. et al. (2010)
**摘要**：通过Western blot和免疫荧光技术(使用Cyclin E2特异性抗体)，揭示Cyclin E2在胰岛素抵抗条件下调控β细胞增殖的机制，为糖尿病治疗提供新靶点。
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如需更多文献或具体应用场景(如实验方法)，可进一步补充说明!
Cyclin E2 is a key regulatory protein involved in cell cycle progression, specifically governing the transition from the G1 phase to the S phase by forming complexes with cyclin-dependent kinases (CDKs), particularly CDK2. It plays a critical role in DNA replication initiation and cell cycle checkpoint control. Dysregulation of Cyclin E2 expression is frequently associated with cancer, where its overexpression can drive uncontrolled cell proliferation, genomic instability, and resistance to apoptosis. This makes Cyclin E2 a biomarker of interest in oncology research and a potential therapeutic target.
Antibodies targeting Cyclin E2 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify protein levels in tissues or cell lines. Specificity and sensitivity are critical, as Cyclin E2 shares structural homology with Cyclin E1. necessitating rigorous validation to avoid cross-reactivity. Researchers often employ knockdown/knockout models or peptide blocking experiments to confirm antibody specificity.
In cancer research, Cyclin E2 antibodies help elucidate mechanisms underlying tumor progression, metastasis, and therapeutic resistance. Elevated Cyclin E2 levels correlate with poor prognosis in cancers like breast, ovarian, and colorectal carcinomas. Additionally, these antibodies aid in developing diagnostic assays and evaluating targeted therapies aimed at restoring cell cycle regulation. Their utility extends to basic research exploring cell cycle dynamics, DNA damage responses, and senescence pathways, underscoring their importance in both translational and fundamental biomedical studies.
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