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Rabbit Polyclonal FLI1 Antibody

  • 中文名: FLI1抗体
  • 别    名: FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB
货号: IPDX22806
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesFLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB
Entrez GeneID2313
WB Predicted band sizeCalculated MW: 51 kDa; Observed MW: 51 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenA synthesized peptide derived from human FLI1
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于FLI1抗体的3篇参考文献的简要总结(基于公开信息及经典研究,具体引用需核实原文):

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1. **文献名称**:*FLI1 as a diagnostic marker for vascular neoplasms: comparison with CD31 and CD34 in a wide spectrum of mesenchymal tumors*

**作者**:Folpe AL, et al.

**摘要**:该研究评估FLI1抗体在血管源性肿瘤(如血管肉瘤、血管瘤)中的诊断价值,发现FLI1相较于传统标志物(CD31、CD34)具有更高的敏感性和特异性,尤其在鉴别低分化血管肉瘤时表现突出。

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2. **文献名称**:*Utility of FLI1 antibody in the diagnosis of Ewing sarcoma and other small round cell tumors*

**作者**:Knoepp SM, et al.

**摘要**:研究验证FLI1抗体在尤文肉瘤(Ewing sarcoma)诊断中的应用,发现其与EWSR1基因易位相关肿瘤高度相关,同时探讨其与其他小圆细胞肿瘤(如横纹肌肉瘤、淋巴瘤)的鉴别诊断作用。

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3. **文献名称**:*FLI1 expression in epithelioid mesothelioma: a potential diagnostic pitfall*

**作者**:Ordonez NG

**摘要**:文章指出FLI1抗体在间皮瘤中可能出现非特异性表达,提示需结合其他标志物(如WT1、calretinin)以避免误诊,强调其在胸膜肺肿瘤鉴别中的局限性。

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4. **文献名称**:*FLI1 overexpression in T-cell acute lymphoblastic leukemia/lymphoma: functional and therapeutic implications*

**作者**:Zhang Y, et al.

**摘要**:研究揭示FLI1在T细胞急性淋巴细胞白血病(T-ALL)中的异常高表达及其促癌机制,为FLI1抗体在白血病分子分型和靶向治疗中的应用提供依据。

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**注意**:以上信息基于领域内代表性研究整理,建议通过PubMed或学术数据库核对具体文献细节及原文内容。

背景信息

The FLI1 antibody is a crucial tool in molecular pathology and research, primarily targeting the Friend Leukemia Integration 1 (FLI1) protein, a member of the ETS family of transcription factors. FLI1 plays pivotal roles in hematopoiesis, vasculogenesis, and oncogenesis, regulating genes involved in cell proliferation, differentiation, and apoptosis. Its expression is predominantly observed in hematopoietic cells (e.g., megakaryocytes, platelets) and endothelial cells. Dysregulation of FLI1 is implicated in various cancers, notably Ewing sarcoma, where a chromosomal translocation t(11;22)(q24;q12) generates the EWSR1-FLI1 fusion oncoprotein, a diagnostic hallmark. FLI1 antibodies are widely used in immunohistochemistry (IHC) to distinguish Ewing sarcoma from other small round cell tumors (e.g., lymphomas, neuroblastomas) due to its nuclear staining pattern. Additionally, FLI1 antibodies aid in studying hematologic malignancies (e.g., erythroleukemia), vascular tumors, and autoimmune diseases like lupus, where FLI1 overexpression in T cells correlates with pathogenesis. Commercial FLI1 antibodies are typically monoclonal (rabbit or mouse) or polyclonal, validated for specificity in IHC, Western blotting, and immunofluorescence. Interpretation requires caution, as FLI1 is expressed in normal tissues (e.g., spleen, lymph nodes) and non-Ewing malignancies (e.g., epithelioid hemangioendothelioma). Ongoing research explores FLI1's therapeutic potential, emphasizing the antibody's diagnostic and experimental utility.

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