| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MDK; FLJ27379; MK1; NEGF2; Midkine; NEGF2; ARAP |
| Entrez GeneID | 4192 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Midkine |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Midkine抗体的3篇参考文献概要(基于公开数据整理，非真实文献)：
---
1. **文献名称**：*Midkine as a novel therapeutic target for cancer: Functional roles of its antibody blockade*
**作者**：Smith J. et al.
**摘要**：研究证明靶向Midkine的单克隆抗体通过抑制肿瘤微环境中的促血管生成和炎症信号通路，显著抑制小鼠模型中结直肠癌的进展，提示其作为癌症免疫治疗的潜在价值。
2. **文献名称**：*Anti-Midkine antibodies attenuate neuropathic pain via modulating microglial activation*
**作者**：Lee S. et al.
**摘要**：该文献发现Midkine抗体通过阻断Midkine与脊髓小胶质细胞表面受体的相互作用，减少促炎因子释放，有效缓解大鼠神经病理性疼痛模型中的痛觉超敏反应。
3. **文献名称**：*Development of a high-affinity Midkine antibody for early diagnosis of acute kidney injury*
**作者**：Wang Y. et al.
**摘要**：研究团队开发了一种新型高亲和力Midkine抗体，可在缺血再灌注损伤动物模型中特异性检测尿液Midkine水平提升，为急性肾损伤的早期无创诊断提供新工具。
---
(注：以上为模拟示例，实际文献需通过PubMed或学术数据库检索确认。)
Midkine (MDK) is a heparin-binding growth factor involved in various cellular processes, including cell proliferation, migration, and angiogenesis. It plays critical roles in embryonic development and tissue repair but is often dysregulated in pathological conditions such as cancer, inflammatory diseases, and neurodegenerative disorders. Structurally, Midkine consists of two domains stabilized by disulfide bonds, enabling interactions with receptors like ALK, LRP1. and integrins, as well as extracellular matrix components.
Midkine antibodies are immunological tools designed to detect, quantify, or inhibit Midkine activity in research and therapeutic contexts. Polyclonal and monoclonal antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and ELISA to study Midkine expression patterns in tissues or biological fluids. In cancer research, these antibodies help elucidate Midkine's role in tumor progression, metastasis, and drug resistance. Therapeutically, neutralizing Midkine antibodies are being explored to block its oncogenic signaling, potentially enhancing chemotherapy efficacy or reducing inflammation in autoimmune diseases. Challenges remain in optimizing antibody specificity and minimizing off-target effects, but ongoing studies highlight Midkine as a promising biomarker and therapeutic target.
×
