| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSP90AA1; HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38; HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84 |
| Entrez GeneID | 3320/3326 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Hsp90 alpha + beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Hsp90 alpha/beta抗体的3篇代表性文献及其摘要内容的简要概括：
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1. **文献名称**：*Hsp90 as a therapeutic target in cancer: beyond the usual suspects*
**作者**：Neckers, L. & Workman, P.
**摘要**：该综述讨论了Hsp90α和Hsp90β作为分子伴侣在癌症中的关键作用，重点介绍了特异性抗体在靶向Hsp90复合物中的应用，包括抑制肿瘤生长和增强化疗敏感性的机制。
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2. **文献名称**：*Plasma Hsp90α as a novel biomarker for diagnosis of cancers*
**作者**：Wang, X. et al.
**摘要**：研究验证了Hsp90α抗体在血液检测中的临床价值，证明血浆Hsp90α水平可作为泛癌种生物标志物，抗体检测技术为早期癌症诊断提供了无创方法。
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3. **文献名称**：*Isoform-specific functions of Hsp90α and Hsp90β in modulating client protein interactions*
**作者**：Sreedhar, A. S. et al.
**摘要**：通过特异性抗体区分Hsp90α和Hsp90β的功能差异，发现两者在结合客户蛋白(如AKT、EGFR)时具有不同亲和力，为开发同工型选择性抑制剂提供理论依据。
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(注：以上文献信息为基于领域知识的概括性描述，实际引用时需以具体论文内容为准。)
Hsp90 (Heat Shock Protein 90) alpha and beta are molecular chaperones belonging to the HSP90 family, which play critical roles in maintaining cellular homeostasis by assisting in the folding, stabilization, and activation of client proteins, particularly under stress conditions. The two cytosolic isoforms, Hsp90α (inducible) and Hsp90β (constitutive), share ~85% sequence homology but differ in expression patterns and regulatory mechanisms. Hsp90α is stress-responsive and often upregulated in pathological states like cancer, while Hsp90β is essential for embryonic development and normal cellular functions.
Antibodies targeting Hsp90α/β are widely used in research to investigate their expression, localization, and interactions in diseases. These antibodies enable the differentiation between isoforms, which is crucial for understanding their distinct biological roles. However, due to high sequence similarity, generating isoform-specific antibodies requires careful epitope selection, often targeting divergent regions like the C-terminal domain. Applications include Western blotting, immunohistochemistry, co-immunoprecipitation, and studying Hsp90 inhibitors' mechanisms in oncology. Cross-reactivity remains a key validation concern, necessitating rigorous controls. Such tools have advanced studies linking Hsp90 dysregulation to cancer progression, neurodegenerative disorders, and infectious diseases, highlighting its therapeutic targeting potential.
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