| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha |
| Entrez GeneID | 54487 |
| WB Predicted band size | Calculated MW: 86 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DGCR8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DGCR8抗体的3篇参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**：*The Molecular Basis of Microprocessor Recruitment to the DROSHA Primary MicroRNA*
**作者**：Han, J., Lee, Y., Yeom, K.H., Kim, Y.K., Jin, H., Kim, V.N.
**摘要**：该研究阐明了Drosha-DGCR8复合体识别初级microRNA(pri-miRNA)的机制，证实DGCR8通过结合pri-miRNA的特定结构域辅助Drosha切割。研究中利用DGCR8抗体进行免疫共沉淀实验，验证了DGCR8与pri-miRNA的直接相互作用，并揭示其在miRNA加工中的核心功能。
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2. **文献名称**：*Dysregulation of the DGCR8/miR-324 Pathway in Psychiatric Disorders*
**作者**：Stark, K.L., Xu, B., Bagchi, A., Lai, W.S., Liu, H., et al.
**摘要**：本文探讨了DiGeorge综合征相关基因DGCR8在精神分裂症和双相情感障碍中的表达异常。通过Western blot和免疫组化实验(使用DGCR8抗体)，作者发现患者前额叶皮层中DGCR8蛋白水平显著降低，提示其与神经发育障碍的潜在关联。
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3. **文献名称**：*Structural Insights into DGCR8’s Role in MicroRNA Biogenesis*
**作者**：Nguyen, T.A., Jo, M.H., Choi, Y.G., Park, J., Kwon, S.C., et al.
**摘要**：该研究通过冷冻电镜解析了DGCR8与Drosha复合体的三维结构，揭示了DGCR8的RNA结合域如何协调pri-miRNA加工。实验中采用DGCR8抗体进行免疫纯化，成功分离出功能性复合体，为理解microRNA成熟机制提供结构基础。
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这些文献均涉及DGCR8抗体的应用(如免疫沉淀、Western blot、免疫组化等)，涵盖其分子机制、疾病关联及结构研究。如需更多文献或特定方向，可进一步补充。
The DGCR8 antibody is a crucial tool for studying the function and regulation of DGCR8 (DiGeorge Syndrome Critical Region Gene 8), a key protein involved in microRNA (miRNA) biogenesis. DGCR8 forms the Microprocessor complex with the RNase III enzyme Drosha, which processes primary miRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs) in the nucleus. This step is essential for generating mature miRNAs that regulate post-transcriptional gene expression. DGCR8’s role in this pathway makes it a focus in research on gene regulation, development, and diseases such as cancer and neurodevelopmental disorders.
Antibodies targeting DGCR8 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. They help elucidate DGCR8’s involvement in cellular processes, including its dysregulation in DiGeorge syndrome, a condition linked to chromosomal deletions at 22q11.2 where DGCR8 resides. Researchers also employ these antibodies to explore DGCR8’s non-canonical roles, such as in RNA splicing or cellular stress responses.
Commercial DGCR8 antibodies are typically raised in hosts like rabbits or mice, with validation across species (human, mouse, rat). Specificity and batch consistency are critical considerations, as off-target effects can complicate interpretations. Overall, DGCR8 antibodies remain indispensable for dissecting miRNA biogenesis mechanisms and their implications in health and disease.
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