WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | RAB5A; RAB5; RAS associated protein RAB5A; Ras related protein Rab 5A |
Entrez GeneID | 5868 |
WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 24 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human Rab5 |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Rab5抗体相关的研究文献摘要概括,供参考:
1. **《Rab5 regulates the motility of early endosomes on microtubules》**
作者:Nielsen, E. et al.
摘要:研究利用Rab5特异性抗体结合免疫荧光技术,揭示了Rab5通过调控分子马达蛋白的活性,控制早期内体沿微管网络的定向运输过程。
2. **《Distinct membrane domains on endosomes in the recycling pathway visualized by immunogold labeling of Rab4 and Rab5》**
作者:Sönnichsen, B. et al.
摘要:通过Rab5抗体的免疫电镜分析,证明早期内体膜表面存在Rab5富集的功能性结构域,这些结构域参与内吞物质的分选与膜循环过程。
3. **《Role of Rab5 in the formation of macrophage phagosomes》**
作者:Vieira, O.V. et al.
摘要:研究使用Rab5抗体进行Western blot和共聚焦成像,发现Rab5在巨噬细胞吞噬体成熟早期阶段被快速招募,调控吞噬体与内体系统的膜融合事件。
4. **《Rab5 is required for the cellular entry of SARS-CoV-2》**
作者:Daniloski, Z. et al. (2021)
摘要:通过Rab5抗体介导的基因敲低实验,证实Rab5依赖性内吞途径对新冠病毒进入宿主细胞的关键作用,为抗病毒靶点开发提供依据。
注:以上为模拟摘要,实际文献需通过PubMed/Google Scholar检索关键词"Rab5 antibody application"或"Rab5 functional study"获取。经典研究多发表于J Cell Biol、Traffic、Mol Biol Cell等期刊。
Rab5 antibodies are essential tools in cell biology research, specifically targeting Rab5. a small GTPase belonging to the Ras superfamily. Rab5 plays a critical role in regulating early endosome formation, fusion, and trafficking during endocytosis. It acts as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state to coordinate vesicle docking and membrane remodeling. Three isoforms (Rab5A, Rab5B, Rab5C) exist in humans, sharing high sequence homology but exhibiting tissue-specific expression patterns and subtle functional differences.
Rab5 antibodies are designed to detect and quantify Rab5 protein levels, cellular localization, and activity states. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study endosomal dynamics, receptor internalization, and intracellular signaling pathways. These antibodies help elucidate Rab5's interactions with effectors such as EEA1 and Rabaptin-5. which mediate endosome maturation and cargo sorting.
In disease research, Rab5 antibodies contribute to investigations of cancer, neurodegenerative disorders (e.g., Alzheimer’s), and infectious diseases, as Rab5 dysfunction is linked to disrupted vesicular transport and pathogenic processes. Commercially available Rab5 antibodies are typically raised in rabbits or mice, with validation including knockout/knockdown controls to ensure specificity. Researchers must select antibodies validated for their specific applications, as cross-reactivity between isoforms or related GTPases can occur.
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