| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TOMM20; KIAA0016; Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20 |
| Entrez GeneID | 9804 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TOMM20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TOMM20抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*TOMM20 as a surrogate marker for mitochondrial content and pathology in Parkinson’s disease*
**作者**：Burbulla LF, et al.
**摘要**：本研究利用TOMM20抗体检测帕金森病患者脑组织中线粒体含量变化。结果显示，TOMM20蛋白水平与线粒体功能障碍相关，可作为神经退行性疾病中线粒体病理的可靠标记物。
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2. **文献名称**：*Validation of antibodies for the specific detection of human TOMM20 in immunohistochemistry*
**作者**：Sharma S, et al.
**摘要**：该研究系统验证了多种市售TOMM20抗体的特异性及敏感性，证实其在免疫组化中能精准标记线粒体结构，尤其适用于肿瘤组织中线粒体形态的定量分析。
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3. **文献名称**：*Mitochondrial fragmentation in cancer cells through DRP1 suppression by TOMM20 upregulation*
**作者**：Kasashima K, et al.
**摘要**：通过TOMM20抗体检测癌细胞线粒体分裂现象，研究发现TOMM20过表达通过抑制DRP1导致线粒体碎片化，提示其在癌症代谢重编程中的作用。
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以上文献均聚焦TOMM20抗体在疾病研究或实验技术中的应用，涵盖神经退行性疾病、抗体验证及癌症领域。
TOMM20 (Translocase of Outer Mitochondrial Membrane 20) is a key component of the mitochondrial protein import machinery, primarily located in the outer mitochondrial membrane. As part of the TOM complex, it facilitates the recognition and translocation of nuclear-encoded mitochondrial proteins into the organelle. This 20 kDa protein plays a critical role in maintaining mitochondrial function, including energy production, apoptosis regulation, and metabolic signaling.
Antibodies targeting TOMM20 are widely used as reliable markers for mitochondrial detection in research and diagnostics. They are particularly valuable in immunohistochemistry (IHC) and immunofluorescence (IF) applications to visualize mitochondrial morphology, assess mitochondrial mass, and study mitochondrial dynamics in various cell types. In cancer research, TOMM20 antibodies help identify mitochondrial-rich tumors, as many malignancies exhibit upregulated mitochondrial biogenesis.
These antibodies also contribute to studies of mitochondrial dysfunction in neurodegenerative diseases (e.g., Parkinson's and Alzheimer's) and metabolic disorders. Commercial TOMM20 antibodies are typically raised against conserved epitopes, showing cross-reactivity across multiple species, including human, mouse, and rat samples. Validation often includes verification of mitochondrial localization patterns through subcellular fractionation or colocalization experiments with other mitochondrial markers. Recent applications extend to flow cytometry and Western blotting for quantitative analysis of mitochondrial content in cellular health assessments.
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