| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASH2; Bre2; ASH2L1; ASH2L2 |
| Entrez GeneID | 9070 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ASH2L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与ASH2L抗体相关的文献及其摘要概述：
1. **"ASH2L mediates histone H3K4 trimethylation to regulate gene silencing in embryonic stem cells"**
*作者：Wang et al.*
**摘要**：研究利用ASH2L特异性抗体进行ChIP-seq分析，发现ASH2L通过调控H3K4me3修饰维持胚胎干细胞关键基因的沉默，揭示其在多能性维持中的作用。
2. **"The role of ASH2L in mixed-lineage leukemia (MLL) complex assembly and leukemogenesis"**
*作者：Chen et al.*
**摘要**：通过免疫共沉淀和Western blot(使用ASH2L抗体)，证明ASH2L是MLL甲基转移酶复合体的核心组分，其缺失破坏H3K4甲基化并抑制白血病细胞的增殖。
3. **"ASH2L promotes tumor progression by enhancing MYC transcription in hepatocellular carcinoma"**
*作者：Li et al.*
**摘要**：采用ASH2L抗体进行免疫组化和ChIP-qPCR，发现ASH2L在肝癌中高表达，通过激活MYC基因的转录驱动肿瘤生长，提示其作为潜在治疗靶点。
这些文献均利用ASH2L抗体探究其在表观遗传调控、疾病机制中的功能，涵盖干细胞、癌症等研究领域。
ASH2L (Absent, Small, or Homeotic-Like) is a core component of the multi-protein histone methyltransferase (HMT) complex, which plays a critical role in regulating gene expression through epigenetic mechanisms. This complex, often associated with the Trithorax group (TrxG), catalyzes the trimethylation of lysine 4 on histone H3 (H3K4me3), a chromatin modification linked to active transcription. ASH2L stabilizes the complex and enhances its catalytic activity by interacting with other subunits like WDR5. RbBP5. and the methyltransferase MLL (Mixed-Lineage Leukemia) or SET1 proteins. It is essential for embryonic development, cell differentiation, and maintaining cell identity by promoting the expression of key developmental genes.
ASH2L antibodies are widely used tools in epigenetics research to study the localization, expression, and function of the ASH2L-containing complex. They enable the detection of ASH2L in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence, helping to map its role in gene regulation and chromatin remodeling. Dysregulation of ASH2L or its complex has been implicated in cancers, developmental disorders, and diseases linked to epigenetic aberrations. For example, aberrant H3K4me3 patterns due to ASH2L dysfunction may contribute to oncogenesis or neurological conditions. Researchers also use these antibodies to explore therapeutic targets by dissecting ASH2L's interactions in disease models. Validated antibodies are critical for specificity, as ASH2L shares structural motifs with other epigenetic regulators. Overall, ASH2L antibodies are indispensable for unraveling the molecular mechanisms of epigenetic regulation and their pathological implications.
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