| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIST1H2AB; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m; H2AFM |
| Entrez GeneID | 3012 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2A.Z |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Histone H2A抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"A monoclonal antibody specific for histone H2A phosphorylated at serine 1"*
**作者**：Fernández-Capetillo, O. et al.
**摘要**：该研究报道了一种针对组蛋白H2A第1位丝氨酸磷酸化(γH2A)的单克隆抗体的开发。作者验证了该抗体在检测DNA损伤应答(如电离辐射诱导的DNA双链断裂)中的特异性，并证明其在免疫荧光和免疫印迹中的应用，为研究染色质损伤信号通路提供了工具。
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2. **文献名称**：*"Histone H2A variants: Diversifying chromatin to ensure genome integrity"*
**作者**：Talbert, P.B. & Henikoff, S.
**摘要**：综述了组蛋白H2A变体(如H2AX、macroH2A)在基因组稳定性和表观遗传调控中的作用。文中总结了多种H2A特异性抗体的应用，例如H2AX磷酸化抗体(γH2AX)在检测DNA修复焦点中的关键作用，并讨论了抗体选择对研究染色质功能的影响。
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3. **文献名称**：*"Antibody-based detection of histone H2A ubiquitination in DNA damage response"*
**作者**：Mattiroli, F. et al.
**摘要**：该研究利用特异性抗体(如抗H2A K119泛素化抗体)揭示了泛素化修饰在DNA损伤修复中的机制。通过ChIP-seq和蛋白质互作分析，作者证明H2A泛素化招募修复复合物(如BRCA1)，并强调了抗体在解析染色质修饰时空动态中的必要性。
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(注：以上文献为示例，实际引用需根据具体研究补充完整信息。)
Histone H2A antibodies are essential tools for studying the structure and function of chromatin, particularly in the context of nucleosome organization and epigenetic regulation. Histone H2A is one of the four core histone proteins (H2A, H2B, H3. H4) that form the histone octamer around which DNA wraps to assemble nucleosomes, the fundamental units of chromatin. H2A plays a critical role in maintaining chromatin stability, DNA repair, and gene expression regulation. Specific post-translational modifications (PTMs) of H2A, such as ubiquitination (e.g., H2AK119ub in mammals) or phosphorylation (e.g., γ-H2AX at Ser139 during DNA damage), are tightly linked to transcriptional silencing, DNA damage response, and epigenetic inheritance.
Antibodies targeting Histone H2A are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting (WB), and immunofluorescence (IF) to investigate chromatin remodeling, histone modification dynamics, and cellular responses to stress. Researchers employ these antibodies to distinguish between unmodified and modified H2A variants, map their genomic localization, or assess their roles in diseases like cancer, where histone dysregulation is common. Specificity is critical, as some antibodies recognize conserved regions across species (human, mouse, rat), while others target PTM-specific epitopes. Validation via knockout/knockdown controls or peptide competition assays ensures reliability. Overall, Histone H2A antibodies are indispensable for unraveling chromatin biology and its implications in health and disease.
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