| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PHB1; HEL-215; HEL-S-54e |
| Entrez GeneID | 5245 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Prohibitin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Prohibitin(PHB)抗体的参考文献及其摘要概述：
1. **文献名称**: "Prohibitin: A role in mitochondria and beyond"
**作者**: Merkwirth C, Langer T
**摘要**: 该文献综述了Prohibitin在线粒体膜结构形成、细胞代谢调控及衰老中的作用，并讨论了PHB抗体在检测线粒体复合物功能中的应用。
2. **文献名称**: "Prohibitin silencing reverses stabilization of mitochondrial integrity and chemoresistance in ovarian cancer cells"
**作者**: Patel N, et al.
**摘要**: 研究利用PHB抗体验证了Prohibitin在卵巢癌细胞化疗耐药性中的作用，表明抑制PHB表达可破坏线粒体稳定性并增强化疗敏感性。
3. **文献名称**: "Prohibitin regulates macrophage function in bacterial infection via TLR signaling"
**作者**: Theiss AL, et al.
**摘要**: 通过PHB抗体的免疫沉淀实验，发现Prohibitin通过与TLR受体互作调控巨噬细胞的炎症反应，影响宿主对细菌感染的免疫应答。
4. **文献名称**: "Antibody-based profiling of subcellular Prohibitin expression in breast cancer"
**作者**: Deng Y, et al.
**摘要**: 该研究使用PHB抗体进行免疫组化分析，揭示了乳腺癌组织中Prohibitin在细胞核和线粒体的差异定位，并关联其表达水平与患者预后。
以上文献均涉及PHB抗体在机制研究或疾病模型中的应用，涵盖癌症、免疫及基础细胞生物学领域。
Prohibitin (PHB) is a highly conserved, ubiquitously expressed protein belonging to the stomatin/prohibitin/flotillin/HflK/C (SPFH) domain family. Initially identified as an antiproliferative protein in mammalian cells, PHB is localized primarily in the mitochondrial inner membrane, where it plays critical roles in mitochondrial homeostasis, including maintaining structural integrity, regulating oxidative phosphorylation, and chaperoning protein folding. It also shuttles to the nucleus, cytoplasm, or cell membrane under specific conditions, participating in diverse cellular processes such as apoptosis, senescence, and transcriptional regulation.
Prohibitin antibodies are essential tools for studying its dual roles in health and disease. Two isoforms, PHB1 (~32 kDa) and PHB2 (~37 kDa), form a heterodimeric complex in mitochondria. Dysregulation of PHB expression has been linked to cancer (acting as tumor suppressors or promoters depending on context), neurodegenerative disorders, and metabolic diseases. Antibodies targeting PHB1/2 are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, subcellular localization, and interactions. Recent studies also explore PHB's potential as a therapeutic target or biomarker, particularly in cancers and aging-related pathologies. Validation of antibody specificity remains crucial due to PHB's homology with other SPFH proteins and variable expression across tissues.
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