| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha |
| Entrez GeneID | 23468 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HP1 alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及HP1α抗体的经典文献，涵盖其功能研究和应用场景：
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1. **文献名称**: *Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins*
**作者**: Lachner, M. et al.
**摘要**: 该研究揭示了HP1蛋白通过其chromo结构域特异性识别组蛋白H3K9me3修饰的分子机制。文中使用HP1α抗体进行免疫荧光和染色质免疫沉淀(ChIP)，证明HP1α通过结合H3K9me3参与异染色质的形成和基因沉默。
2. **文献名称**: *Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain*
**作者**: Bannister, A.J. et al.
**摘要**: 本文通过结构生物学和生化实验，证明HP1α的chromo结构域直接结合H3K9me3标记。研究中利用HP1α抗体进行免疫共沉淀(Co-IP)和Western blot，验证了HP1α与组蛋白甲基转移酶SUV39H1的功能联系。
3. **文献名称**: *Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin*
**作者**: Thiru, A. et al.
**摘要**: 该研究解析了HP1α与核蛋白(如CAF1)相互作用的晶体结构，揭示其通过PXVXL基序招募其他异染色质调控因子。实验中使用HP1α抗体进行免疫荧光定位，证实其在细胞核内异染色质区域的特异性富集。
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**备注**：上述文献均为真实研究，但具体标题和内容细节可能需要通过PubMed或Google Scholar进一步确认。HP1α抗体常用于染色质动态、表观遗传学及癌症相关研究中。
**Background of HP1α Antibody**
Heterochromatin Protein 1 alpha (HP1α), encoded by the *CBX5* gene, is a conserved epigenetic regulator critical for maintaining heterochromatin structure and gene silencing. HP1α binds to histone H3 lysine 9 trimethylation (H3K9me3) via its chromodomain, facilitating chromatin compaction and transcriptional repression. It plays key roles in genome stability, telomere maintenance, and cell cycle regulation.
HP1α antibodies are essential tools for studying heterochromatin dynamics, nuclear organization, and epigenetic mechanisms. They enable detection and localization of HP1α in techniques like immunofluorescence, Western blotting, and ChIP-seq. Research using these antibodies has linked HP1α dysfunction to diseases, including cancer (via aberrant gene silencing) and aging (through heterochromatin loss).
Originally identified in *Drosophila*, HP1 proteins (α, β, γ) are evolutionarily conserved, with HP1α being predominant in constitutive heterochromatin. Studies leveraging HP1α antibodies have advanced understanding of epigenetic inheritance, DNA repair, and cellular differentiation. Commercial HP1α antibodies are validated for specificity, often targeting epitopes within the chromo- or hinge domains, ensuring reliable application across experimental models.
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