| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98 Nup96; Nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; Nup98; Nup98-Nup96; NUP98_HUMAN. |
| Entrez GeneID | 4928 |
| WB Predicted band size | Calculated MW: 198 kDa; Observed MW: 188 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NUP98 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NUP98抗体的参考文献示例(内容为模拟概括，仅供参考)：
1. **《NUP98 Fusion Oncoproteins Promote Aneuploidy by Impairing the Mitotic Checkpoint》**
- 作者：Slape C et al.
- 摘要：研究NUP98融合蛋白在急性髓系白血病(AML)中引发染色体不稳定的机制，通过NUP98抗体进行免疫沉淀和ChIP-seq实验，揭示其干扰有丝分裂检查点的分子途径。
2. **《Nuclear Pore Complex Protein NUP98 Is Required for Embryonic Stem Cell Differentiation》**
- 作者：Hase ME et al.
- 摘要：利用NUP98特异性抗体进行免疫荧光和Western blot，证明NUP98在小鼠胚胎干细胞分化过程中调控核质运输及基因表达的关键作用。
3. **《Altered NUP98 Localization in Myelodysplastic Syndromes with 11p15 Aberrations》**
- 作者：Wang X et al.
- 摘要：通过免疫组化(IHC)结合NUP98抗体，分析骨髓增生异常综合征(MDS)患者样本中NUP98的异常核定位，揭示其与疾病进展的相关性。
4. **《A Novel NUP98 Antibody Validates Its Use as a Biomarker in Leukemia Diagnostics》**
- 作者：Kimura S et al.
- 摘要：开发并验证一种高特异性NUP98单克隆抗体，应用于流式细胞术和免疫印迹，提升NUP98相关白血病融合基因的临床检测灵敏度。
(注：以上文献为示例，实际引用需以真实出版物为准。)
The NUP98 antibody targets the NUP98 protein, a key component of the nuclear pore complex (NPC) that regulates nucleocytoplasmic transport of mRNA and proteins. NUP98 contains repetitive phenylalanine-glycine (FG) motifs critical for its interaction with transport receptors. It is frequently implicated in chromosomal translocations, particularly in hematologic malignancies, where fusion with over 30 partner genes (e.g., HOXA9. NSD1) generates oncoproteins driving leukemogenesis. These fusions disrupt normal NPC function and transcriptional regulation, often conferring poor prognosis.
NUP98 antibodies are essential tools for detecting wild-type NUP98 or its fusion variants in research and diagnostics. They are widely used in techniques like Western blot, immunofluorescence, and immunohistochemistry to study localization, expression levels, and interactions in diseases. In leukemia, these antibodies help identify NUP98 rearrangements via fluorescence in situ hybridization (FISH) or immunoprecipitation, aiding subtype classification and therapeutic strategies.
Recent studies also explore NUP98's roles beyond transport, including chromatin interactions and gene regulation. However, antibody specificity remains a challenge due to shared epitopes in fusion proteins. Ongoing research focuses on developing isoform-specific antibodies and leveraging NUP98 fusion-driven pathways for targeted therapies, highlighting its dual role as a cellular scaffold and oncogenic driver.
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