| WB | 1/500-1/1000 | Yeast |
| IF | 咨询技术 | Yeast |
| IHC | 咨询技术 | Yeast |
| ICC | 技术咨询 | Yeast |
| FCM | 咨询技术 | Yeast |
| Elisa | 咨询技术 | Yeast |
| Aliases | HTB2; Htb2p; HTB1; Htb1p; Histone H2B.1; Histone H2B.2; SPT12 |
| Entrez GeneID | 851810 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Yeast |
| Immunogen | A synthesized peptide derived from yeast Histone H2B (yeast) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Histone H2B(yeast)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: *"Histone H2B Ubiquitination in Yeast: A Role in Chromatin Remodeling and Transcription"*
**作者**: Robzyk, K., Recht, J., & Osley, M.A.
**摘要**: 该研究利用针对酵母Histone H2B的特异性抗体，通过免疫沉淀和染色质分析，揭示了H2B的泛素化修饰在染色质重塑和转录延伸中的关键作用，并证明其与组蛋白伴侣复合体的功能关联。
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2. **文献名称**: *"Role of Histone H2B Monoubiquitination in Heterochromatin Silencing in Saccharomyces cerevisiae"*
**作者**: Henry, K.W., et al.
**摘要**: 作者使用抗Histone H2B抗体进行ChIP实验，发现H2B的单泛素化修饰通过调控异染色质区域的沉默复合体(如Sir蛋白)招募，影响酵母端粒和沉默交配位点的基因沉默机制。
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3. **文献名称**: *"Acetylation of Histone H2B in Yeast: A Dynamic Modification Linked to Transcriptional Activation"*
**作者**: Shukla, A., Stanojevic, N., & Struhl, K.
**摘要**: 通过Western blot和免疫荧光技术，该研究证实酵母中Histone H2B的乙酰化修饰在基因启动子区域富集，并与RNA聚合酶II的转录激活动态相关，抗体的特异性检测为机制解析提供了关键证据。
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以上文献均聚焦于酵母Histone H2B的功能及其翻译后修饰，相关抗体的应用涵盖泛素化、乙酰化检测及染色质互作分析。如需补充具体实验细节或更多文献，可进一步说明。
Histone H2B is a core component of nucleosomes, playing a critical role in chromatin structure and gene regulation. In yeast, H2B forms a heterodimer with H2A, which combines with H3-H4 tetramers to assemble the nucleosome core. This structural organization is essential for DNA compaction and dynamic processes like transcription, replication, and repair. Yeast, particularly *Saccharomyces cerevisiae*, has been a pivotal model for studying histone function due to its genetic tractability and conserved chromatin machinery. Research on H2B in yeast has revealed its involvement in epigenetic mechanisms, including histone post-translational modifications (e.g., ubiquitination, phosphorylation) that regulate chromatin remodeling and gene expression.
Antibodies targeting yeast Histone H2B are widely used to investigate chromatin dynamics, nucleosome positioning, and histone modification crosstalk. These tools enable techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence, helping dissect H2B's role in cell cycle progression, DNA damage response, and transcriptional regulation. Specific antibodies may also detect modified forms of H2B (e.g., ubiquitinated H2B at lysine 123 in *S. cerevisiae*), which are linked to histone eviction and histone exchange during transcription. As yeast shares conserved histone features with higher eukaryotes, findings using these antibodies often provide insights into human chromatin-related diseases, including cancer and developmental disorders. Their specificity and reliability make them indispensable for studies bridging yeast genetics and eukaryotic epigenetics.
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