| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) |
| Entrez GeneID | 3312 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Hsc70 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Hsc70 regulates the interaction of Alzheimer's disease-related proteins"**
- 作者：Smith A, et al.
- 摘要：探讨Hsc70在阿尔茨海默病相关蛋白(如tau和β-淀粉样蛋白)相互作用中的作用，通过免疫共沉淀和Western blot技术，验证Hsc70抗体在检测其与病理蛋白结合中的特异性。
2. **"Characterization of Hsc70 in cellular stress response using monoclonal antibodies"**
- 作者：Lee J, et al.
- 摘要：开发并验证一种新型Hsc70单克隆抗体，用于免疫荧光和流式细胞术，揭示Hsc70在热休克条件下向细胞核的转位及其应激保护机制。
3. **"Role of Hsc70 in cancer cell autophagy: Insights from antibody-based assays"**
- 作者：Chen R, et al.
- 摘要：利用Hsc70抗体进行免疫组化和蛋白质印迹分析，证明Hsc70通过调控自噬小体形成促进肿瘤细胞存活，为靶向治疗提供依据。
4. **"Distinct functions of Hsc70 and Hsp70 revealed by antibody specificity profiling"**
- 作者：Garcia-Mata R, et al.
- 摘要：通过比较多种Hsc70/Hsp70抗体的交叉反应性，明确区分两者在蛋白质折叠和细胞定位中的差异，强调抗体选择对功能研究的重要性。
(注：以上文献为示例性内容，实际引用需根据具体研究补充真实文献信息。)
Hsc70 (heat shock cognate 70 kDa protein) is a constitutively expressed member of the HSP70 (heat shock protein 70) family, functioning as a molecular chaperone involved in protein folding, intracellular trafficking, and the stabilization of misfolded proteins under stress conditions. Unlike its stress-inducible counterpart Hsp70. Hsc70 is abundantly present in most cells under normal physiological conditions. It plays critical roles in maintaining cellular proteostasis, facilitating clathrin-coated vesicle uncoating, and assisting in antigen presentation through interactions with MHC class I/II molecules.
Antibodies targeting Hsc70 are essential tools for studying its expression patterns, subcellular localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate Hsc70's involvement in neurodegenerative diseases (e.g., Alzheimer's), cancer progression, and viral infection mechanisms. Specificity is crucial due to high sequence homology (over 85%) between Hsc70 and other HSP70 family members. Most commercial antibodies are raised against unique epitopes in Hsc70's N-terminal ATPase domain or C-terminal substrate-binding domain, with validation often involving knockout cell lines.
Research applications frequently focus on Hsc70's dual role in protein quality control – promoting either refolding or degradation of client proteins through co-chaperone partnerships. Its antibody-based detection also aids in exploring therapeutic strategies targeting chaperone networks in protein aggregation disorders and chemotherapy resistance.
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