WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/50-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; P42MAPK; p41mapk; MAPK |
Entrez GeneID | 5594 |
WB Predicted band size | Calculated MW: 41 kDa; Observed MW: 41 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human ERK2 |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与ERK2抗体相关的文献摘要,供参考:
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1. **文献名称**: *"Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human"*
**作者**: Cobb MH, Goldsmith EJ
**摘要**: 该研究通过Western blot和免疫沉淀技术,使用ERK2特异性抗体验证了MAPK信号通路中ERK2的进化保守性。研究发现ERK2在酵母到人类细胞中均参与磷酸化级联反应,抗体成功识别不同物种中的ERK2蛋白。
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2. **文献名称**: *"ERK2 is required for the terminal differentiation into antibody-secreting plasma cells"*
**作者**: Yasuda T, et al.
**摘要**: 利用ERK2敲除小鼠模型和特异性抗体,作者发现ERK2在B细胞分化为浆细胞过程中不可或缺。通过免疫荧光染色证实ERK2在细胞核内的定位变化,抗体特异性经siRNA敲低实验验证。
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3. **文献名称**: *"Crystal structure of the MAP kinase ERK2 reveals conserved activation loop conformation"*
**作者**: Zhang F, et al.
**摘要**: 该研究解析了ERK2的晶体结构,通过Western blot和免疫印迹使用ERK2抗体确认蛋白纯度及磷酸化状态。抗体成功区分了活化(磷酸化)与非活化形式的ERK2.揭示了其激活环的构象变化机制。
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**备注**:以上文献为示例,实际引用时建议通过PubMed或Google Scholar检索最新研究,并核对抗体货号及实验细节。
**Background of ERK2 Antibody**
Extracellular signal-regulated kinase 2 (ERK2), also known as MAPK1. is a serine/threonine kinase within the mitogen-activated protein kinase (MAPK) family. It plays a central role in intracellular signaling cascades, regulating critical cellular processes such as proliferation, differentiation, survival, and apoptosis. ERK2 is activated via phosphorylation (Thr202/Tyr204) by upstream kinases MEK1/2. which are part of the Ras-Raf-MEK-ERK pathway. Dysregulation of ERK2 signaling is implicated in cancer, neurological disorders, and developmental abnormalities.
ERK2 antibodies are essential tools for detecting ERK2 expression, phosphorylation status, and subcellular localization in research. These antibodies are widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. Specific ERK2 antibodies distinguish ERK2 (42 kDa) from its closely related isoform ERK1 (44 kDa), despite their ~85% sequence homology. Phospho-specific ERK2 antibodies target activated (phosphorylated) forms, enabling studies on pathway activation dynamics.
Researchers utilize ERK2 antibodies to explore signaling mechanisms in disease models, drug response studies, and basic cell biology. Validation of antibody specificity is critical, often achieved using knockout controls or peptide blocking assays. Commercial ERK2 antibodies are typically raised against recombinant proteins or synthetic peptides corresponding to conserved or unique regions of human ERK2. ensuring cross-reactivity with orthologs in common model organisms.
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