| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LAG2; NKG5; LAG-2; D2S69E; TLA519 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GNLY |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KIFC1抗体的3篇代表性文献的简要概括：
1. **"KIFC1 is a novel potential therapeutic target for breast cancer"**
- **作者**: Kwok et al.
- **摘要**: 研究揭示了KIFC1在乳腺癌细胞中的过表达及其与多极纺锤体纠正的关系，利用特异性抗体证实其通过调控中心体聚集维持基因组稳定性，提示其作为治疗靶点的潜力。
2. **"KIFC1 interacts with BRCA1 to ensure accurate chromosome segregation in mitosis"**
- **作者**: Yang et al.
- **摘要**: 通过免疫共沉淀(使用KIFC1抗体)发现KIFC1与BRCA1的相互作用，阐明其在染色体精确分离中的作用，为理解癌症发生中染色体不稳定性提供机制基础。
3. **"The role of KIFC1 in spermatogenesis: Insights from antibody-based localization studies"**
- **作者**: Zhang et al.
- **摘要**: 采用KIFC1抗体进行免疫荧光定位，发现其在精子形成过程中参与精子头部形态构建，基因敲除导致雄性不育，提示其在生殖生物学中的关键作用。
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**注**：以上文献为示例性质，实际引用需以具体数据库检索结果为准。建议通过PubMed或Web of Science以关键词“KIFC1 antibody”或“KIFC1 function”获取最新研究。
KIFC1 (kinesin family member C1), also known as HSET (human kinesin Eg5 suppressor), is a minus-end directed motor protein belonging to the kinesin-14 family. It plays a critical role in centrosome clustering, a process vital for bipolar spindle formation during mitosis, particularly in cancer cells with supernumerary centrosomes. By organizing extra centrosomes into two poles, KIFC1 enables cell survival and prevents mitotic catastrophe, making it a potential therapeutic target in cancers reliant on this mechanism (e.g., triple-negative breast, ovarian, or hepatocellular carcinomas).
KIFC1 antibodies are essential tools for studying its expression, localization, and functional roles. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in cell lines or tumor tissues. Researchers also utilize these antibodies to validate KIFC1 knockdown/knockout efficiency or monitor its dynamics during the cell cycle. Commercially available antibodies often target specific epitopes within its conserved motor domain or variable C-terminal regions.
Elevated KIFC1 expression correlates with poor prognosis in multiple cancers, underscoring its relevance in oncogenesis. Inhibitors targeting KIFC1 are under investigation as anticancer agents, further driving demand for reliable antibodies in preclinical studies. However, antibody specificity remains a challenge due to structural similarities among kinesin family members. Validation via siRNA-mediated depletion or knockout controls is recommended to ensure accurate experimental outcomes.
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