| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha |
| Entrez GeneID | 5578 |
| WB Predicted band size | Calculated MW: 77 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PKC alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PKCα抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Protein kinase C alpha: a multifunctional regulator of cell plasticity*
**作者**：Mauro, L.V., et al.
**摘要**：该研究综述了PKCα在肿瘤发生中的作用，利用特异性抗体证实其在调控细胞增殖、凋亡及迁移中的关键功能，并探讨其作为癌症治疗靶点的潜力。
2. **文献名称**：*Production and characterization of a monoclonal antibody against human PKC alpha*
**作者**：Newton, A.C., et al.
**摘要**：本文详细描述了针对人源PKCα单克隆抗体的开发过程，通过免疫印迹和免疫荧光验证其高特异性，并应用于细胞定位及信号通路研究。
3. **文献名称**：*PKC alpha-dependent phosphorylation in Alzheimer’s disease pathology*
**作者**：Battaini, F., et al.
**摘要**：研究利用PKCα抗体发现，阿尔茨海默病患者脑组织中PKCα活性降低，其异常磷酸化与tau蛋白病理沉积相关，提示其在神经退行性疾病中的调节作用。
(注：以上文献信息为示例性概括，实际引用需核对原文准确性。)
**Background of PKC Alpha Antibody**
Protein kinase C alpha (PKCα), a member of the classical PKC subfamily, is a serine/threonine kinase critical for regulating diverse cellular processes, including proliferation, apoptosis, differentiation, and migration. It requires calcium ions and diacylglycerol (DAG) for activation, functioning as a key mediator in signal transduction pathways triggered by growth factors, hormones, and other extracellular stimuli. Structurally, PKCα contains regulatory (C1 and C2 domains) and catalytic (kinase) regions, enabling its role in phosphorylating downstream targets like MAPK and NF-κB.
PKCα antibodies are essential tools for studying its expression, localization, and activity in normal and diseased tissues. These antibodies enable detection via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding research into PKCα's dysregulation in cancers (e.g., breast, colon, and glioblastoma), where it often promotes tumor progression and drug resistance. Additionally, PKCα is implicated in cardiovascular diseases, diabetes, and neurological disorders, making its antibodies valuable for mechanistic and therapeutic studies.
Commercial PKCα antibodies are typically developed using immunogens from conserved regions (e.g., C-terminus) and validated for specificity to avoid cross-reactivity with other PKC isoforms. Their applications extend to drug discovery, particularly in evaluating PKCα inhibitors as potential therapies. Understanding PKCα's context-dependent roles—pro-survival or pro-apoptotic—remains a research focus, underscoring the importance of reliable antibodies in dissecting its complex biology.
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