| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 1/50-1/200 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JAK 1A; JAK1A; JNK 1; JNK 46; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; MAPK8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; PRKM 8; PRKM8; Protein kinase JNK1; Protein kinase; mitogen-activated; 8; SAPK 1; SAPK gamma; SAPK1; Stress activated protein kinase JNK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1; Tyrosine protein kinase JAK1 . |
| Entrez GeneID | 5599 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 46,54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | Recombinant protein of human JNK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于JNK抗体的参考文献，按文献名称、作者和摘要内容简要整理：
1. **文献名称**：*Signal Transduction by the JNK Group of MAP Kinases*
**作者**：Davis, R.J.
**摘要**：该综述总结了JNK(c-Jun N-terminal kinase)信号通路的分子机制，包括其在细胞应激反应、凋亡和炎症中的作用。文中强调了特异性JNK抗体的开发和应用，用于检测不同亚型(JNK1、JNK2、JNK3)的激活状态(如磷酸化水平)。
2. **文献名称**：*The JNK Pathway: A Therapeutic Target in Neurological Disorders*
**作者**：Borsello, T., & Forloni, G.
**摘要**：研究探讨JNK通路在神经退行性疾病(如阿尔茨海默病)中的调控作用，并利用JNK特异性抗体(如抗p-JNK)验证其在脑组织样本中的激活，为靶向治疗提供实验依据。
3. **文献名称**：*Differential Roles of JNK1 and JNK2 in Regulating CD8+ T Cell Responses*
**作者**：Tournier, C., et al.
**摘要**：通过亚型特异性JNK抗体(JNK1 vs JNK2)的Western blot和流式细胞术分析，揭示了JNK1和JNK2在T细胞免疫应答中的不同功能，强调抗体特异性对实验结果的重要性。
4. **文献名称**：*Antibody-Based Profiling of JNK Phosphorylation Dynamics in Cancer Cells*
**作者**：Sabio, G., & Davis, R.J.
**摘要**：研究开发了一种基于JNK磷酸化特异性抗体的检测方法，实时追踪肿瘤细胞中JNK活性变化，并验证其与化疗耐药性的关联，为癌症治疗提供新思路。
以上文献均涉及JNK抗体的实验应用(如亚型区分、磷酸化检测)，覆盖基础机制研究和疾病模型分析。如需具体期刊信息或页码，可进一步补充关键词或研究领域缩小范围。
The c-Jun N-terminal kinase (JNK) antibody is a crucial tool in studying the JNK signaling pathway, which belongs to the mitogen-activated protein kinase (MAPK) family. JNKs (JNK1. JNK2. JNK3) are serine/threonine kinases activated by stress signals, cytokines, or growth factors, regulating cellular processes like apoptosis, proliferation, inflammation, and differentiation. JNK1 and JNK2 are ubiquitously expressed, while JNK3 is primarily found in the brain and testes. Antibodies targeting JNK isoforms or their phosphorylated (activated) forms (e.g., p-JNK) enable researchers to detect expression levels, activation status, and subcellular localization in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, or immunofluorescence.
These antibodies are pivotal in studying diseases linked to JNK dysregulation, such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and inflammatory conditions. For example, elevated p-JNK levels correlate with tumor progression or neuronal apoptosis. Specificity is critical, as cross-reactivity between isoforms or non-specific binding can lead to inaccurate interpretations. Commercial JNK antibodies are often validated using knockout controls or peptide-blocking assays to ensure reliability. Researchers must select antibodies based on target species, isoform specificity, and application compatibility. Overall, JNK antibodies serve as indispensable reagents for unraveling the complex roles of JNK signaling in health and disease.
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