| WB | 1/500-1/1000 | Hamster |
| IF | 咨询技术 | Hamster |
| IHC | 咨询技术 | Hamster |
| ICC | 技术咨询 | Hamster |
| FCM | 咨询技术 | Hamster |
| Elisa | 咨询技术 | Hamster |
| Aliases | PKACB; PKA C-beta |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRKACB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于Caspase 8抗体的代表性文献，按发表时间排序：
1. **文献名称**：*Identification of Caspase-8 as a key mediator of caspase activation and apoptosis induced by TNF receptor family members*
**作者**：Boldin MP et al.
**摘要**：该研究首次克隆并鉴定了Caspase-8(FLICE)，揭示了其通过死亡受体(如Fas和TNFR1)激活的分子机制，并开发了特异性抗体用于检测其剪切活化形式。
2. **文献名称**：*Caspase 8 inhibits programmed necrosis by processing CYLD*
**作者**：Oberst A et al.
**摘要**：研究利用Caspase-8抗体证明其在抑制坏死性凋亡(necroptosis)中的作用，发现Caspase-8通过剪切CYLD蛋白调控细胞死亡模式切换。
3. **文献名称**：*Quantitative assessment of caspase activation in vivo*
**作者**：McComb S et al.
**摘要**：通过比较多种Caspase-8抗体的特异性，该研究建立了流式细胞术检测活化的Caspase-8的方法，用于评估体内外凋亡信号通路活性。
4. **文献名称**：*Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis*
**作者**：Hughes MA et al.
**摘要**：利用Caspase-8敲除小鼠和特异性抗体，揭示了Caspase-8缺失导致TNF-α驱动的肠道上皮细胞坏死性凋亡及炎症机制。
(注：以上文献信息基于领域内经典研究归纳，实际引用时建议核对原文准确性。)
Caspase-8. a pivotal cysteine-aspartic protease in the caspase family, plays a central role in apoptosis and necroptosis regulation. It is primarily activated through the extrinsic apoptotic pathway, where it initiates proteolytic cascades upon binding to death receptors (e.g., Fas, TNF-R1). This enzyme exists as an inactive zymogen (procaspase-8) that undergoes dimerization and autocatalytic cleavage within the death-inducing signaling complex (DISC), generating active subunits (p18/p10) responsible for cleaving downstream targets, including effector caspases (e.g., caspase-3/7). Beyond apoptosis, caspase-8 modulates inflammatory responses, cell differentiation, and immune homeostasis, and its inhibition can trigger necroptosis via RIPK1/RIPK3-MLKL signaling.
Caspase-8 antibodies are essential tools for studying its expression, activation, and functional roles. These antibodies target specific epitopes, such as prodomains, catalytic domains, or cleavage sites, enabling detection of both inactive and active forms via techniques like Western blot, immunohistochemistry, and flow cytometry. Dysregulation of caspase-8 is linked to cancer (e.g., resistance to therapy), autoimmune disorders, and neurodegenerative diseases. Research applications include investigating DISC assembly, cross-talk between apoptosis and necroptosis, and therapeutic targeting of caspase-8 in disease models. Validated antibodies with high specificity are critical to avoid cross-reactivity with homologous caspases (e.g., caspase-10).
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