| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCNB1; CCNB; G2/mitotic-specific cyclin-B1 |
| Entrez GeneID | 268697 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of mouse Cyclin B1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclin B1抗体的3篇参考文献的简要列举：
1. **"Cyclin B1 is a prognostic biomarker in breast cancer"**
- **作者**: Yuan, J. et al.
- **摘要**: 该研究通过免疫组化(IHC)分析Cyclin B1在乳腺癌组织中的表达，发现其高表达与患者预后不良显著相关，提示Cyclin B1可作为潜在的肿瘤预后标志物。
2. **"Antibody specificity in cell cycle analysis: A comparative study of Cyclin B1 antibodies"**
- **作者**: Brandeis, M. & Hunt, T.
- **摘要**: 文章比较了多种Cyclin B1抗体的特异性，通过Western blot和免疫荧光验证了抗体在检测细胞周期依赖性Cyclin B1表达变化中的可靠性，为实验选择提供参考。
3. **"Cyclin B1 degradation and antibody-based detection in synchronized cells"**
- **作者**: Porter, L.A. & Donoghue, D.J.
- **摘要**: 研究利用Cyclin B1抗体分析细胞周期同步化过程中蛋白的表达及降解机制，揭示了其在有丝分裂退出时的动态变化，强调抗体在细胞周期研究中的关键作用。
4. **"Cyclin B1 misregulation in viral oncogenesis"**
- **作者**: Hsu, C.H. et al.
- **摘要**: 探讨HPV感染中病毒蛋白对Cyclin B1表达的干扰，通过抗体共聚焦成像显示Cyclin B1亚细胞定位异常，为病毒致癌机制提供新视角。
(注：以上文献信息为概括性示例，实际引用需核实具体来源。)
Cyclin B1 is a critical regulatory protein involved in cell cycle progression, specifically controlling the transition from the G2 phase to mitosis (M phase). It forms a complex with cyclin-dependent kinase 1 (CDK1), activating its kinase activity to drive mitotic entry. Cyclin B1 levels fluctuate during the cell cycle, peaking in late G2 phase before rapidly degrading via the ubiquitin-proteasome system during mitosis. Dysregulation of Cyclin B1 expression is associated with uncontrolled cell proliferation, genomic instability, and cancer progression, making it a biomarker of interest in oncology research.
Antibodies targeting Cyclin B1 are essential tools for studying its expression, localization, and function in cellular and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to quantify Cyclin B1 levels in tissues or cultured cells. Specificity and validation are crucial, as Cyclin B1 shares structural homology with other cyclin family members. High-quality antibodies distinguish between phosphorylated or unmodified forms and detect subcellular shifts (e.g., cytoplasmic to nuclear translocation during mitosis).
In cancer research, Cyclin B1 antibodies help assess tumor aggressiveness, therapeutic response, and prognosis, as overexpression often correlates with poor clinical outcomes. Additionally, they aid in exploring Cyclin B1's role in checkpoint regulation and its potential as a therapeutic target. Researchers must optimize experimental conditions, considering cell cycle synchronization and sample fixation methods to ensure accurate detection.
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