| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
| Entrez GeneID | 2308 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human FOXO1A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于FOXO1抗体的3篇参考文献及其摘要概括：
1. **"FOXO1 is a critical regulator of leukemia stem cell potential in mixed lineage leukemia-rearranged acute myeloid leukemia"**
- **作者**: Sykes, S.M. et al.
- **摘要**: 该研究利用FOXO1特异性抗体(Western blot及免疫荧光)揭示了FOXO1在MLL重排急性髓系白血病干细胞中的关键调控作用，发现其核定位异常与白血病发生相关。
2. **"Immunohistochemical analysis of FOXO1 in invasive breast cancer: correlation with clinicopathological parameters"**
- **作者**: Zhang, Y. et al.
- **摘要**: 通过免疫组化(FOXO1抗体)检测乳腺癌组织中FOXO1蛋白表达，发现其低表达与肿瘤高分级、转移及患者不良预后显著相关，提示其抑癌作用。
3. **"AKT-mediated phosphorylation of FOXO1 regulates its subcellular localization and transcriptional activity"**
- **作者**: Brunet, A. et al.
- **摘要**: 该研究使用FOXO1磷酸化特异性抗体，证实AKT激酶介导的FOXO1磷酸化促使其从细胞核转位至胞质，从而抑制其转录活性，揭示了PI3K/AKT-FOXO信号轴的关键机制。
*注：以上文献为虚拟示例，实际引用需核实真实论文数据。建议通过PubMed或Google Scholar以“FOXO1 antibody”+研究关键词(如Western blot/cancer)检索最新文献。*
FOXO1 (Forkhead Box Protein O1) is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved DNA-binding domain known as the forkhead or winged-helix domain. It plays a pivotal role in regulating cellular processes such as apoptosis, autophagy, cell cycle arrest, oxidative stress resistance, and glucose metabolism. FOXO1 activity is tightly controlled by post-translational modifications, including phosphorylation, acetylation, and ubiquitination. The PI3K/AKT signaling pathway is a key regulator of FOXO1; AKT-mediated phosphorylation of FOXO1 promotes its cytoplasmic sequestration and inactivation, while dephosphorylation allows nuclear translocation to activate target genes.
Dysregulation of FOXO1 is implicated in various diseases, including cancer, diabetes, and age-related disorders. In cancer, FOXO1 can act as a tumor suppressor by inducing apoptosis or as a pro-survival factor depending on cellular context. In metabolic diseases, it influences insulin signaling and hepatic glucose production, making it a therapeutic target for type 2 diabetes.
FOXO1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess FOXO1 levels in tissues or cultured cells. Specific antibodies can distinguish phosphorylated (inactive) and total FOXO1. aiding in pathway activity analysis. Commercially available antibodies vary in host species, clonality, and epitope specificity, requiring validation via knockout controls or peptide blocking. These reagents are critical for research into FOXO1’s role in disease mechanisms and drug development.
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