| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4 |
| Entrez GeneID | 581 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Bax |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Bax抗体相关的文献摘要信息：
1. **文献名称**: "Specificity of Commercial Antibodies Against Bax: Implications for Apoptosis Studies"
**作者**: Zhiyuan Zhang et al.
**摘要**: 该研究评估了多种市售Bax抗体的特异性，通过Western blot和免疫组化验证其在检测人/小鼠细胞中内源性Bax蛋白时的交叉反应性，发现部分抗体存在非特异性结合，强调了抗体验证在细胞凋亡研究中的必要性。
2. **文献名称**: "Pro-apoptotic BAX and BAK: A Requisite Gateway to Mitochondrial Dysfunction in Apoptosis"
**作者**: Stanley J. Korsmeyer et al.
**摘要**: 文章阐明了Bax蛋白在线粒体途径凋亡中的核心作用，通过基因敲除模型证明Bax/Bak双缺失细胞对凋亡信号抵抗，并讨论了利用特异性抗体定位Bax激活构象变化的技术进展。
3. **文献名称**: "Bax Translocation to Mitochondria in Parkinson's Disease Brain"
**作者**: Miquel Vila et al.
**摘要**: 该研究使用Bax特异性抗体在帕金森病患者脑组织中发现Bax蛋白向线粒体的显著转位，提示其参与多巴胺能神经元凋亡，为神经退行性疾病的机制提供了实验依据。
*注：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar核对最新权威研究。*
Bax antibodies are essential tools in apoptosis research, targeting the Bax protein—a pro-apoptotic member of the Bcl-2 family. Bax plays a critical role in mitochondrial outer membrane permeabilization (MOMP), a pivotal step in intrinsic apoptosis. Under normal conditions, Bax resides in the cytosol in an inactive conformation. Upon cellular stress (e.g., DNA damage), activated Bax translocates to mitochondria, oligomerizes, and induces cytochrome *c* release, triggering caspase activation and apoptosis. Dysregulation of Bax is linked to cancer, neurodegeneration, and autoimmune diseases.
Bax antibodies are widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to detect Bax expression, localization, and activation status. They help differentiate between inactive (monomeric) and active (mitochondrial-bound) Bax, providing insights into apoptotic pathways. Commercially available clones (e.g., 2D2. 6A7) vary in specificity for conformational epitopes or post-translational modifications. Researchers select antibodies based on applications, species reactivity (human, mouse, rat), and compatibility with fixation methods. Validation via knockout controls ensures reliability. Bax antibodies are crucial for studying apoptosis mechanisms, drug screening (e.g., chemotherapeutics that enhance Bax activation), and understanding disease pathogenesis linked to apoptotic dysregulation.
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