| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK. |
| Entrez GeneID | 5595/5594 |
| WB Predicted band size | Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human ERK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases"** (作者: Robinson MJ, Cobb MH; *Science*, 1997)
摘要：该综述详细阐述了ERK1/2信号通路的生物学功能及其在细胞增殖、分化和应激反应中的作用，强调了ERK1/2抗体的应用在检测其活化状态(如磷酸化形式)中的关键意义。
2. **"A novel role for ERK1/2 as a negative regulator of the c-Jun N-terminal kinase pathway"** (作者: Cargnello M, et al.; *Cell*, 2013)
摘要：研究通过Western blot和免疫沉淀实验，利用ERK1/2特异性抗体揭示了ERK1/2对JNK通路的抑制作用，证明了抗体在解析交叉信号通路调控机制中的必要性。
3. **"Selective activation of ERK1/2 by protein kinase A in human cancer cells"** (作者: Yao Z, et al.; *J Biol Chem*, 2012)
摘要：该文献通过磷酸化特异性ERK1/2抗体，验证了PKA在特定肿瘤细胞中选择性激活ERK1/2的机制，强调了抗体在区分总蛋白与活化蛋白中的技术优势。
4. **"ERK1/2 promotes neuronal differentiation through phosphorylation of the TrkB receptor"** (作者: Frödin M, et al.; *EMBO J*, 2002)
摘要：研究利用ERK1/2抗体及磷酸化抗体，结合神经元模型阐明了ERK1/2通过磷酸化TrkB受体调控神经分化的分子机制，展示了抗体在细胞功能研究中的关键作用。
ERK1/2 (extracellular signal-regulated kinases 1 and 2), also known as MAPK3 and MAPK1. are serine/threonine protein kinases central to the RAS-RAF-MEK-ERK signaling pathway. This evolutionarily conserved cascade regulates diverse cellular processes, including proliferation, differentiation, survival, and apoptosis. ERK1 (44 kDa) and ERK2 (42 kDa) share ~85% sequence homology and are activated through dual phosphorylation at conserved threonine and tyrosine residues (Thr202/Tyr204 in ERK1; Thr185/Tyr187 in ERK2) by upstream MEK1/2 kinases. Their activity is triggered by extracellular stimuli such as growth factors, hormones, and stress signals.
ERK1/2 antibodies are essential tools for studying this pathway's activation status and localization. Most antibodies target either the phosphorylated (active) form or total ERK1/2 protein. Phospho-specific antibodies recognize the dually phosphorylated activation loop, enabling detection of pathway activation, while pan-ERK antibodies identify both active and inactive forms. Some antibodies distinguish between ERK1 and ERK2 isoforms, though many detect both due to their high homology. These reagents are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry. Validation parameters include specificity confirmation via knockout controls and functional assays. Dysregulation of ERK1/2 signaling is implicated in cancer, inflammatory diseases, and neurological disorders, making these antibodies crucial for both basic research and drug development targeting this pathway.
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