| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E4; UFD2; HDNB1; UBOX3; UFD2A |
| Entrez GeneID | 10277 |
| WB Predicted band size | Calculated MW: 146 kDa; Observed MW: 146 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human UBE4B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Ubiquitin Conjugation Factor E4 B(UBE4B)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **"UBE4B interacts with the ITCH E3 ubiquitin ligase to regulate degradation of tumor suppressor p73"**
- **作者**：Wang et al.
- **摘要**：研究揭示了UBE4B通过结合ITCH E3泛素连接酶调控肿瘤抑制因子p73的泛素化及降解，实验中采用抗UBE4B抗体进行免疫共沉淀(Co-IP)验证蛋白相互作用。
2. **"UBE4B deficiency enhances protein aggregate clearance through increased autophagy"**
- **作者**：Zhang et al.
- **摘要**：本文发现UBE4B缺失通过激活自噬途径促进异常蛋白聚集体的清除，研究利用UBE4B特异性抗体进行Western blot和免疫荧光分析，证实其在神经退行性疾病模型中的表达变化。
3. **"The role of UBE4B in DNA damage response and genomic stability"**
- **作者**：Johnson et al.
- **摘要**：该文献阐明了UBE4B在DNA损伤修复中的功能，通过抗UBE4B抗体检测其在细胞核内的定位，并证明其与BRCA1复合物的关联，影响基因组稳定性。
以上研究均依赖UBE4B抗体完成关键实验，涵盖蛋白互作、降解机制及疾病模型分析。如需更多文献或具体DOI，可进一步补充检索。
Ubiquitin Conjugation Factor E4 B (UBE4B), also known as UFD2a, is a critical enzyme within the ubiquitin-proteasome system, responsible for mediating the attachment of ubiquitin molecules to substrate proteins. As an E4 ubiquitin ligase, UBE4B facilitates the elongation of polyubiquitin chains, a process essential for targeting specific proteins for degradation by the proteasome. This enzyme plays a pivotal role in maintaining cellular protein homeostasis, regulating processes such as DNA repair, cell cycle progression, and the clearance of misfolded proteins. Dysregulation of UBE4B has been implicated in neurodegenerative diseases, cancer, and other pathologies linked to impaired ubiquitination.
Antibodies targeting UBE4B are vital tools for investigating its expression, localization, and functional interactions. These antibodies enable researchers to detect endogenous UBE4B protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They are also used in co-immunoprecipitation assays to study UBE4B’s binding partners and its role in ubiquitination cascades. High-quality UBE4B antibodies are rigorously validated for specificity, often using knockout cell lines or siRNA-mediated knockdown to confirm target selectivity. Such reagents contribute to understanding UBE4B’s dual roles as both a tumor suppressor and potential oncogene, depending on cellular context, and its involvement in diseases like Alzheimer’s and Parkinson’s. Ongoing research aims to clarify its regulatory mechanisms and therapeutic potential.
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