| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A |
| Entrez GeneID | 7337 |
| WB Predicted band size | Calculated MW: 101 kDa; Observed MW: 101 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human UBE3A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 Ubiquitin Protein Ligase E3A (UBE3A) 抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*"UBE3A/E6-AP regulates cell proliferation by promoting proteasomal degradation of p27"*
**作者**：Kishino T., Lalande M., Wagstaff J.
**摘要**：该研究揭示了UBE3A通过泛素-蛋白酶体系统调控细胞周期蛋白p27的降解，从而影响细胞增殖。研究使用特异性抗体验证了UBE3A与p27的相互作用，并证明UBE3A缺失导致p27积累和细胞周期停滞。
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2. **文献名称**：*"Imprinted expression of the murine Angelman syndrome gene Ube3a in hippocampal and Purkinje neurons"*
**作者**：Albrecht U., Sutcliffe J.S., Beaudet A.L.
**摘要**：本文通过免疫组织化学和Western blot实验，利用UBE3A特异性抗体证明小鼠大脑中该基因的母系特异性表达模式，特别是在海马体和小脑浦肯野细胞中，为Angelman综合征的神经病理机制提供依据。
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3. **文献名称**：*"Antibody-based profiling of brain ubiquitin proteasome system components in Angelman syndrome"*
**作者**：Mandel-Brehm C., et al.
**摘要**：研究开发了一种针对UBE3A的高特异性抗体，用于分析Angelman综合征患者脑组织样本中UBE3A蛋白的分布及表达水平变化，揭示了其与神经元泛素化底物异常的关联。
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如需更多文献或具体应用场景(如抗体克隆号、实验方法等)，可进一步补充检索条件。
The ubiquitin protein ligase E3A (UBE3A), also known as E6AP, is a key enzyme in the ubiquitin-proteasome system, responsible for tagging target proteins with ubiquitin molecules to regulate their degradation, localization, or activity. UBE3A is particularly notable for its genomic imprinting in neurons, where only the maternal allele is expressed in the brain. Loss-of-function mutations or deletions in the maternal UBE3A gene cause Angelman syndrome, a severe neurodevelopmental disorder characterized by intellectual disability, speech impairment, and motor dysfunction. Conversely, overexpression of UBE3A has been linked to autism spectrum disorders and certain cancers, highlighting its critical role in neurodevelopment and cellular homeostasis.
Antibodies targeting UBE3A are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate UBE3A's function in both physiological and pathological contexts. These antibodies often recognize specific domains of UBE3A, such as its HECT (homologous to E6AP C-terminus) domain, which is crucial for its ligase activity. Research applications include exploring UBE3A's involvement in synaptic plasticity, protein turnover, and its interplay with viral oncoproteins like HPV E6. Validating antibody specificity is critical, as UBE3A shares structural homology with other E3 ligases, and off-target binding can compromise experimental outcomes.
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