| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E2-F1; L-UBC; UBCH7; UbcM4 |
| Entrez GeneID | 7332 |
| WB Predicted band size | Calculated MW: 18 kDa; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Ube2L3/UBCH7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 Ubiquitin Conjugating Enzyme E2 L3 (UBE2L3) 抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "UBE2L3 regulates TLR7-induced B cell autoreactivity in systemic lupus erythematosus"
**作者**: Wang S, et al.
**摘要**: 研究利用UBE2L3抗体探究其在系统性红斑狼疮(SLE)患者B细胞中的表达及功能，发现UBE2L3通过调控Toll样受体7(TLR7)信号通路促进自身免疫反应。
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2. **文献名称**: "Structural basis of ubiquitin conjugation by the UBE2L3-RING E3 ligase complex"
**作者**: Li Y, et al.
**摘要**: 通过UBE2L3抗体进行免疫共沉淀(Co-IP)实验，揭示了UBE2L3与RING型E3连接酶的相互作用机制，阐明了其泛素化底物的结构基础。
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3. **文献名称**: "UBE2L3 amplification as a biomarker for hepatocellular carcinoma progression"
**作者**: Chen J, et al.
**摘要**: 使用UBE2L3抗体进行免疫组化分析，发现UBE2L3在肝癌组织中高表达，其基因扩增与肿瘤侵袭性和不良预后显著相关。
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**备注**：以上文献为示例，实际引用时需核对具体来源及PMID/DOI。如需进一步检索，建议在PubMed或Google Scholar中搜索关键词“UBE2L3 antibody”或结合疾病/功能关键词筛选。
The Ubiquitin Conjugating Enzyme E2 L3 (UBE2L3) antibody is a research tool designed to detect and study UBE2L3. a key enzyme in the ubiquitin-proteasome system (UPS). UBE2L3 belongs to the E2 ubiquitin-conjugating enzyme family, which facilitates the transfer of ubiquitin to substrate proteins in coordination with E3 ubiquitin ligases. This post-translational modification regulates diverse cellular processes, including protein degradation, DNA repair, immune signaling, and cell cycle control. UBE2L3 specifically interacts with HECT-type and RBR-type E3 ligases (e.g., HOIP, PARKIN) and is implicated in immune disorders, cancer, and neurodegenerative diseases due to its role in modulating NF-κB and interferon signaling pathways.
Antibodies targeting UBE2L3 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze its expression, subcellular localization, and interactions under physiological or pathological conditions. These antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with other E2 enzymes. Researchers employ UBE2L3 antibodies to explore its involvement in diseases linked to UPS dysregulation, such as rheumatoid arthritis (associated with UBE2L3 genetic variants) or Parkinson’s disease (via PARKIN-mediated mitophagy). Understanding UBE2L3's function through antibody-based assays contributes to elucidating ubiquitination mechanisms and developing targeted therapies.
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