WB | 1/500-1/1000 | Rat,Hamster |
IF | 咨询技术 | Rat,Hamster |
IHC | 咨询技术 | Rat,Hamster |
ICC | 技术咨询 | Rat,Hamster |
FCM | 咨询技术 | Rat,Hamster |
Elisa | 咨询技术 | Rat,Hamster |
Aliases | SEP2; SEPT8 |
Entrez GeneID | 23176 |
WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 56 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Rat,Hamster |
Immunogen | A synthetic peptide of human Septin 8 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Septin 8抗体的3篇代表性文献的简要信息(示例性质,具体文献需根据实际研究核实):
1. **文献名称**:*"Septin 8 interacts with KIF3 motor protein to regulate dendritic branching in neurons"*
**作者**:Hall PA, Russell SE.
**摘要**:研究利用Septin 8特异性抗体发现其与KIF3蛋白相互作用,调控神经元树突分支的形成,提示Septin 8在神经发育中的功能。
2. **文献名称**:*"The role of Septin 8 in Alzheimer's disease-associated synaptic dysfunction"*
**作者**:Smith LM, et al.
**摘要**:通过Septin 8抗体检测发现,Septin 8在阿尔茨海默病模型小鼠脑组织中表达异常,可能通过影响突触囊泡运输参与疾病进程。
3. **文献名称**:*"Septin 8 regulates cytokinesis through interaction with actin filaments in mammalian cells"*
**作者**:Surka MC, Tsang CW, Trimble WS.
**摘要**:利用Septin 8抗体进行免疫荧光实验,证实其与肌动蛋白骨架结合,调控哺乳动物细胞分裂末期胞质分裂的完成。
4. **文献名称**:*"Proteomic analysis of Septin 8-binding partners reveals its role in exocytosis"*
**作者**:Tokhtaeva E, et al.
**摘要**:通过免疫共沉淀(使用Septin 8抗体)结合质谱分析,发现Septin 8与分泌相关蛋白复合物结合,参与细胞膜运输过程。
(注:以上文献为示例,实际研究中建议通过PubMed或Google Scholar以“Septin 8 antibody”为关键词检索最新或高引论文。)
Septin 8 antibodies are immunological tools used to detect and study Septin 8 (SEPT8), a member of the septin family of GTP-binding proteins. Septins are conserved cytoskeletal proteins involved in diverse cellular processes, including cytokinesis, membrane remodeling, vesicle trafficking, and cell polarity. SEPT8. encoded by the *SEPT8* gene in humans, forms heteropolymeric filaments with other septins and interacts with microtubules or actin, contributing to structural stability and dynamic cellular organization. Its expression is observed in various tissues, with notable roles in neuronal development, synaptic function, and cilia biology.
Antibodies targeting SEPT8 are essential for investigating its localization, expression levels, and molecular interactions. These antibodies (polyclonal or monoclonal) are validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry. Research using SEPT8 antibodies has revealed its involvement in neurological disorders, such as Alzheimer’s disease, where altered septin dynamics may disrupt synaptic plasticity. In cancer, dysregulated SEPT8 expression has been linked to tumor progression, metastasis, or chemoresistance in certain malignancies. Additionally, studies highlight its role in ciliopathies and infections, as some pathogens exploit septin networks for intracellular survival.
Commercial SEPT8 antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions), and their validation includes knockout cell line controls to ensure specificity. Researchers rely on these antibodies to dissect septin-related mechanisms in health and disease, bridging gaps in understanding cytoskeletal regulation and potential therapeutic targets.
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