| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SD; Fug1; RANGAP |
| Entrez GeneID | 5905 |
| WB Predicted band size | Calculated MW: 64 kDa; Observed MW: 68,82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human RanGAP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RanGAP1抗体的3篇参考文献示例(基于经典研究，建议通过PubMed或Google Scholar核对最新文献)：
1. **"The nucleoporin Nup358 associates with and regulates interphase microtubules"**
- **作者**: Joseph J, et al. (2002)
- **摘要**: 研究利用RanGAP1抗体揭示了其在有丝分裂过程中与纺锤体微管的相互作用，并发现其磷酸化修饰可能调控其动态定位。
2. **"SUMO modification of RanGAP1 and its role in nuclear transport"**
- **作者**: Zhang H, Clarke P. (2000)
- **摘要**: 通过RanGAP1抗体检测SUMO化修饰对其核膜定位的影响，证明SUMO化是RanGAP1结合核孔复合体的关键步骤。
3. **"Characterization of RanGAP1-containing complexes in interphase and mitosis"**
- **作者**: Matunis MJ, et al. (1996)
- **摘要**: 早期利用RanGAP1抗体进行免疫沉淀和免疫荧光定位，发现其与核孔蛋白Nup358的关联，支持其在核质运输中的功能。
4. **"Importin β negatively regulates nuclear membrane fusion and nuclear pore complex assembly"**
- **作者**: Nemergut ME, et al. (2001)
- **摘要**: 使用RanGAP1抗体研究其在核膜重建中的作用，发现importin β通过结合RanGAP1调控核孔复合体组装。
**注**：以上为示例文献，实际引用需确认具体研究内容及抗体应用场景(如物种来源、实验方法等)。建议通过数据库补充近年研究(如癌症中RanGAP1异常表达的抗体分析)。
RanGAP1 (Ran GTPase-Activating Protein 1) is a critical regulatory component of the Ran GTPase cycle, which governs nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation. As a GTPase-activating protein, RanGAP1 accelerates the hydrolysis of Ran-bound GTP to GDP, establishing the RanGTP-RanGDP gradient essential for directional transport across the nuclear pore complex. It is evolutionarily conserved and typically localizes to the nuclear envelope and spindle microtubules during mitosis.
Antibodies targeting RanGAP1 are widely used to study its roles in cellular processes. In mammals, RanGAP1 is post-translationally modified by SUMOylation, which directs its association with the nuclear pore complex. Researchers employ RanGAP1 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, subcellular distribution, and interaction partners. These antibodies have been instrumental in elucidating mitotic errors, nuclear transport defects, and links to diseases such as cancer, where RanGAP1 dysregulation may contribute to genomic instability.
Commercially available RanGAP1 antibodies are often validated in model organisms (e.g., Arabidopsis, human, yeast), reflecting its conserved function. Controls using knockout cells or competitive peptides are recommended due to potential cross-reactivity with related proteins.
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