| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P47; Platelet 47 kDa protein |
| Entrez GeneID | 5341 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Pleckstrin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Pleckstrin抗体相关的文献示例(部分信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *Pleckstrin homology domain-mediated membrane translocation and binding of phosphoinositides by phospholipase C-δ1*
**作者**: Yagisawa H, et al.
**摘要**: 本研究利用Pleckstrin抗体探针，验证了PLC-δ1蛋白的PH结构域与细胞膜磷脂酰肌醇磷酸(PIPs)的特异性结合机制，揭示了其在胞内信号转导中的关键定位功能。
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2. **文献名称**: *Characterization of a monoclonal antibody against pleckstrin homology domain of protein kinase B*
**作者**: Jiang T, Li S.
**摘要**: 作者开发了一种针对Akt/PKB蛋白PH结构域的单克隆抗体，通过免疫印迹和免疫荧光实验验证其特异性，证实该抗体可用于检测细胞中Akt的亚细胞定位及激活状态。
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3. **文献名称**: *Pleckstrin regulates platelet functional responses via phosphoinositide 3-kinase-dependent and -independent pathways*
**作者**: Rondina MT, et al.
**摘要**: 通过Pleckstrin抗体阻断实验，研究发现Pleckstrin蛋白在血小板活化过程中通过PI3K依赖和非依赖的双重通路调控细胞骨架重组与分泌功能。
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4. **文献名称**: *Development of a novel polyclonal antibody for pleckstrin and its application in detecting immune disorders*
**作者**: Chen L, et al.
**摘要**: 研究团队制备了一种兔源多克隆Pleckstrin抗体，经ELISA和免疫组化验证，发现其在系统性红斑狼疮患者外周血单核细胞中表达异常，提示Pleckstrin可能参与自身免疫病理过程。
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建议通过PubMed或Web of Science以“pleckstrin antibody”及“PH domain antibody”为关键词检索最新文献，或查阅《Journal of Biological Chemistry》《Blood》等期刊相关研究。
Pleckstrin, encoded by the *PLEK* gene, is a ubiquitously expressed protein initially identified in platelets and leukocytes. It functions as a critical regulator of cellular signaling, particularly in immune cells and during cytoskeletal reorganization. Pleckstrin contains two pleckstrin homology (PH) domains, which mediate interactions with membrane phospholipids and other signaling molecules, and a central DEP (Dishevelled, Egl-10. Pleckstrin) domain. Its activity is tightly regulated by phosphorylation, primarily via protein kinase C (PKC), which triggers conformational changes enabling interactions with downstream effectors. Pleckstrin plays roles in processes like phagocytosis, chemotaxis, and platelet activation, linking receptor signaling to cytoskeletal dynamics.
Pleckstrin antibodies are essential tools for studying its expression, localization, and post-translational modifications. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and flow cytometry to investigate Pleckstrin's involvement in immune responses, inflammation, and thrombotic disorders. Research has also implicated Pleckstrin dysregulation in pathologies like autoimmune diseases, cancer metastasis, and cardiovascular disorders, driving interest in targeting its pathways therapeutically. Antibodies specific to phosphorylated forms (e.g., at Ser113/Ser137) help dissect PKC-dependent signaling mechanisms. Validation across species and applications ensures their utility in both basic and translational studies, underscoring their importance in cellular and molecular research.
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