| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCF; CGD3; P40PHOX; SH3PXD4 |
| Entrez GeneID | 4689 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human p40-phox |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NCF4抗体的3篇参考文献示例，涵盖其功能、疾病关联及实验应用：
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1. **文献名称**：*"NCF4 phosphorylation regulates NADPH oxidase assembly and activation in human neutrophils"*
**作者**：Dang, P. M., et al.
**摘要**：该研究通过免疫沉淀和Western blot技术，利用NCF4特异性抗体揭示了NCF4的磷酸化修饰在NADPH氧化酶复合体组装中的关键作用，证明其缺陷会导致活性氧(ROS)生成减少，影响中性粒细胞的杀菌功能。
2. **文献名称**：*"Role of NCF4 in chronic granulomatous disease: Insights from a murine knockout model"*
**作者**：Jackson, S. H., et al.
**摘要**：通过构建NCF4基因敲除小鼠模型，结合NCF4抗体检测蛋白表达，研究发现NCF4缺失导致NADPH氧化酶活性受损，引发类似慢性肉芽肿病的免疫缺陷表型，强调其在宿主防御中的必要性。
3. **文献名称**：*"TLR4-induced ROS-dependent inflammatory signaling requires NCF4 and p47phox interaction in macrophages"*
**作者**：Matsushima, H., et al.
**摘要**：研究利用NCF4抗体进行共聚焦显微镜分析，发现TLR4激活后，NCF4与p47phox的相互作用促进ROS产生，驱动巨噬细胞炎症反应，为靶向NCF4调控过度炎症提供了依据。
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以上文献示例展示了NCF4抗体在分子机制、疾病模型及信号通路研究中的应用，涵盖基础科学和临床相关性。如需具体文献，建议通过PubMed或Web of Science以关键词检索获取全文。
The NCF4 antibody targets the Neutrophil Cytosolic Factor 4 (NCF4), a critical subunit of the NADPH oxidase complex, which plays a central role in innate immunity. NCF4. also known as p40phox, is part of a family of cytosolic proteins (including p47phox, p67phox, and Rac) that interact with membrane-bound components (gp91phox and p22phox) to form the active NADPH oxidase. This enzyme generates reactive oxygen species (ROS) essential for microbial killing and inflammatory signaling. NCF4 contains an SH3 domain that mediates protein-protein interactions, facilitating oxidase assembly and activation.
Mutations in NCF4 or dysregulation of NADPH oxidase function are linked to chronic granulomatous disease (CGD), characterized by impaired ROS production and recurrent infections. Additionally, NCF4 has been implicated in autoimmune and inflammatory disorders, such as rheumatoid arthritis, due to its role in regulating ROS-dependent signaling pathways.
NCF4 antibodies are vital tools for studying protein expression, localization, and interactions in immune cells. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate NADPH oxidase dynamics, cellular responses to pathogens, and mechanisms of oxidative stress. Researchers also employ these antibodies to explore NCF4's involvement in inflammatory pathways and its potential as a therapeutic target. Understanding NCF4's function through antibody-based assays contributes to advancements in immunology, infection biology, and the development of treatments for immune-related diseases.
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