| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EOPA; MITAP1; NDEL1; NUDEL |
| Entrez GeneID | 81565 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human NDEL1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NDEL1抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**: *NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation and maintenance*
**作者**: Mori D, Yamada M, Mimori-Kiyosue Y, et al.
**摘要**: 研究揭示了Aurora-A激酶通过磷酸化NDEL1调控中心体成熟与分离的机制，利用NDEL1抗体验证其在有丝分裂中的定位和功能。
2. **文献名称**: *Role of NDEL1 in neurodevelopmental disorders: insights from schizophrenia-associated genetic variants*
**作者**: Ozeki Y, Tomoda T, Kleiderlein J, et al.
**摘要**: 通过NDEL1抗体检测患者脑组织样本，发现NDEL1表达异常与精神分裂症相关基因变异存在关联，提示其在神经发育障碍中的作用。
3. **文献名称**: *NDEL1 regulates dynein recruitment to microtubule plus-ends and coordinates neuronal migration*
**作者**: Moon HM, Wynshaw-Boris A.
**摘要**: 利用NDEL1抗体进行免疫沉淀和活细胞成像，证明NDEL1通过调控动力蛋白在微管末端的募集，影响神经元迁移的时空协调性。
4. **文献名称**: *Structural basis for NDEL1 interaction with Lis1 and cytoplasmic dynein*
**作者**: Torisawa T, Toyoshima Y, Nishida Y, et al.
**摘要**: 结合X射线晶体学和NDEL1抗体标记实验，阐明了NDEL1与Lis1及动力蛋白复合体的结构互作机制，揭示其在细胞分裂中的分子基础。
以上文献均涉及NDEL1抗体在分子机制、疾病模型或结构研究中的应用，可供快速参考。
NDEL1 (Nuclear Distribution Element-Like 1) is a critical protein involved in neuronal development, cell division, and microtubule dynamics. It interacts with LIS1 (PAFAH1B1) and dynein/dynactin complexes to regulate mitotic spindle orientation, neuronal migration, and cortical layering during brain development. NDEL1 also plays roles in neurogenesis, axon elongation, and synaptic function. Its dysregulation is linked to neurodevelopmental disorders like lissencephaly, schizophrenia, and Alzheimer’s disease.
NDEL1 antibodies are essential tools for studying these biological processes and disease mechanisms. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect NDEL1 expression, localization, and interactions in cells and tissues. Specific epitopes targeted by these antibodies often reside in NDEL1’s N-terminal coiled-coil domains or C-terminal regions, which mediate binding to LIS1 and dynein. Many NDEL1 antibodies are raised against recombinant protein fragments or synthetic peptides, with validation in knockout models to ensure specificity.
Research applications include investigating NDEL1’s role in neurodevelopmental pathways, microtubule stabilization, and centrosome positioning. These antibodies also aid in exploring NDEL1’s dual functions in both mitosis (via dynein regulation) and post-mitotic neuronal maturation, bridging developmental biology and neuroscience studies.
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