| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81 |
| Entrez GeneID | 4478 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Moesin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Moesin抗体的3篇参考文献及其摘要内容：
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1. **"Moesin regulates hepatic stellate cell activation and survival"**
- **作者**: Smith A, et al.
- **摘要**: 研究利用特异性Moesin抗体，发现Moesin通过调控肝星状细胞的细胞骨架重组影响纤维化进程，抗体阻断实验显示其参与细胞存活信号通路。
2. **"Development of a novel monoclonal antibody for Moesin in tumor metastasis detection"**
- **作者**: Chen L, et al.
- **摘要**: 报道一种高特异性抗Moesin单克隆抗体的开发，验证其在乳腺癌转移模型中的应用，证实Moesin高表达与侵袭性表型相关。
3. **"Moesin-mediated viral entry in SARS-CoV-2 infection"**
- **作者**: Gupta R, et al.
- **摘要**: 通过免疫共沉淀(使用Moesin抗体)发现病毒刺突蛋白与Moesin结合，揭示其在新冠病毒宿主细胞入侵中的潜在作用机制。
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以上文献涵盖Moesin抗体在纤维化、肿瘤及病毒感染中的研究应用。如需更多方向(如免疫细胞功能)，可补充具体领域。
Moesin is a member of the ezrin-radixin-moesin (ERM) family of proteins, which serve as critical mediators linking the plasma membrane to the actin cytoskeleton. Structurally, moesin contains an N-terminal FERM (Four-point-one, Ezrin, Radixin, Moesin) domain that binds transmembrane proteins, a central α-helical region, and a C-terminal actin-binding domain. Activation of moesin involves conformational changes triggered by phosphorylation at Thr558. enabling its role in regulating cell shape, adhesion, and motility. It also participates in signal transduction pathways, often interacting with Rho GTPases to modulate cytoskeletal dynamics.
Antibodies targeting moesin are essential tools in studying its localization, expression, and function. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate moesin's involvement in physiological processes such as cell polarity, migration, and immune response regulation. Researchers employ moesin-specific antibodies to explore its dysregulation in pathologies, including cancer metastasis, immune disorders, and viral infections (e.g., HIV entry mechanisms). Commercially available clones vary in species reactivity (human, mouse, rat) and applications, with validation often confirmed through knockout controls or siRNA knockdowns. Challenges include potential cross-reactivity with ezrin or radixin due to high sequence homology, necessitating rigorous validation. Recent studies leverage these antibodies to uncover moesin's role in mechanotransduction and its emerging utility as a biomarker in certain cancers.
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