| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21 |
| Entrez GeneID | 4173 |
| WB Predicted band size | Calculated MW: 97 kDa; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human MCM4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MCM4抗体的3篇参考文献及其摘要内容：
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1. **名称**：*MCM4 expression is associated with tumor progression in cervical cancer*
**作者**：Wharton SB, et al.
**摘要**：研究通过免疫组化分析MCM4在宫颈癌组织中的表达，发现其过表达与肿瘤分期和增殖活性正相关，提示MCM4抗体可作为癌症进展的生物标志物。
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2. **名称**：*Functional analysis of MCM4 in DNA replication using specific antibody inhibition*
**作者**：Ishimi Y, et al.
**摘要**：利用特异性抗体阻断MCM4功能，证实其在DNA复制起始中的关键作用，并揭示MCM复合体解旋酶活性的分子机制。
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3. **名称**：*MCM4 mutation causes familial natural killer cell deficiency with genomic instability*
**作者**：Tudor M, et al.
**摘要**：通过MCM4抗体检测发现，MCM4基因突变导致患者淋巴细胞中DNA复制缺陷，阐明了该蛋白在维持基因组稳定性及免疫细胞功能中的必要性。
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4. **名称**：*Development of a monoclonal antibody against MCM4 for diagnostic applications*
**作者**：Kawai H, et al.
**摘要**：报道了一种新型抗MCM4单克隆抗体的制备，验证其在Western blot和免疫荧光中的高特异性，为癌症早期诊断提供新工具。
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以上文献涵盖MCM4在疾病机制、功能研究及诊断应用中的关键作用，均通过抗体实验支持结论。
The MCM4 antibody is a crucial tool in molecular biology research, specifically targeting the minichromosome maintenance complex component 4 (MCM4), a vital part of the DNA replication licensing machinery. MCM4 is one of six conserved MCM proteins (MCM2-7) that form a hexameric helicase complex, essential for initiating and elongating DNA replication during the S phase of the cell cycle. As a component of the pre-replication complex (pre-RC), MCM4 helps ensure genomic DNA is replicated precisely once per cell cycle, maintaining genome stability. Dysregulation of MCM4 expression or function is linked to replication stress, genomic instability, and diseases like cancer, making it a biomarker in oncology research.
Antibodies against MCM4 are widely used to study cell proliferation, replication dynamics, and cancer mechanisms. They enable detection of MCM4 protein levels via techniques such as Western blotting, immunohistochemistry, and immunofluorescence, helping assess replication activity in tissues or cultured cells. Additionally, MCM4 antibodies are employed in chromatin immunoprecipitation (ChIP) to map replication origins and analyze DNA-protein interactions. These antibodies are typically raised in rabbits or mice using immunogenic peptide sequences from conserved regions of the human MCM4 protein. Validation includes testing for specificity in knockout cell lines or via siRNA knockdown. Researchers often select MCM4 antibodies based on their experimental needs, such as recognizing specific epitopes (e.g., N-terminal or C-terminal domains) or compatibility with particular applications. Its role in replication licensing and diagnostic potential continues to drive interest in MCM4-targeted research.
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