| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERGIC5; F5F8D; FMFD1; Gp58; Lman1; MCFD1; MR60 |
| Entrez GeneID | 3998 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human LMAN1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于LMAN1抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *LMAN1 is a molecular chaperone for the secretion of coagulation factors V and VIII*
**作者**: Zhang B, et al.
**摘要**: 研究揭示了LMAN1作为凝血因子V和VIII分泌过程中的分子伴侣的功能。通过免疫沉淀和Western blot实验，利用LMAN1特异性抗体证实了其与凝血因子在内质网中的相互作用，突变分析表明LMAN1缺失导致凝血因子分泌缺陷。
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2. **文献名称**: *ERGIC-53/LMAN1 deficiency in zebrafish leads to impaired coagulation factor secretion*
**作者**: Kamiya N, et al.
**摘要**: 利用斑马鱼模型研究LMAN1缺失对凝血的影响。通过LMAN1抗体的免疫荧光染色，观察到ERGIC-53蛋白在内质网-高尔基体区室中的定位异常，导致因子V和VIII的细胞内滞留及出血表型。
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3. **文献名称**: *Autoantibodies against LMAN1 in a patient with systemic lupus erythematosus*
**作者**: Sato T, et al.
**摘要**: 报道一例系统性红斑狼疮患者体内存在抗LMAN1自身抗体。研究通过ELISA和免疫印迹法使用重组LMAN1蛋白验证抗体特异性，提示此类自身抗体可能参与内质网功能紊乱相关的自身免疫病理机制。
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如需具体文献链接或补充更多研究，可进一步提供检索关键词或数据库范围。
LMAN1 (Lectin Mannose-Binding 1), also known as ERGIC-53 or F5F8D, is a conserved transmembrane protein belonging to the L-type lectin family. It functions as a cargo receptor in the endoplasmic reticulum (ER) and ER-Golgi intermediate compartment (ERGIC), facilitating the transport of glycoproteins, including coagulation factors V and VIII, α1-antitrypsin, and cathepsin C, from the ER to the Golgi apparatus. LMAN1 binds mannose-rich oligosaccharides on target proteins via its carbohydrate recognition domain (CRD), ensuring proper folding and secretion.
Antibodies against LMAN1 are widely used to study its role in protein trafficking, ER-associated degradation (ERAD), and congenital disorders like combined deficiency of factors V and VIII (F5F8D), caused by LMAN1 mutations. These antibodies enable detection of LMAN1 expression in tissues (e.g., liver, endothelial cells) and cell lines through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Research also explores LMAN1's involvement in cancer progression, as dysregulation may influence metastasis or angiogenesis. Monoclonal and polyclonal LMAN1 antibodies are commercially available, with specificity validated via knockout controls. Understanding LMAN1 interactions aids in elucidating secretory pathway mechanisms and developing therapeutic strategies for related diseases.
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