| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | HMG 2; HMG B2; HMG-2; HMG2; HMGB2 |
| Entrez GeneID | 3148 |
| WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | A synthetic peptide of human HMGB2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与HMGB2抗体相关的代表性文献(内容为虚构示例，实际文献需通过数据库查询)：
1. **标题**：*HMGB2 promotes hepatocellular carcinoma progression by regulating PD-L1 expression*
**作者**：Chen L, et al.
**摘要**：本研究利用HMGB2特异性抗体，通过免疫组化和Western blot分析，发现HMGB2在肝癌组织中高表达，并通过调控PD-L1介导免疫逃逸，提示其作为肝癌治疗靶点的潜力。
2. **标题**：*HMGB2 maintains stemness of colorectal cancer stem cells via Wnt/β-catenin signaling*
**作者**：Wang Y, et al.
**摘要**：通过CRISPR敲低HMGB2及抗体介导的染色质免疫沉淀(ChIP)，证明HMGB2通过激活Wnt通路维持结直肠癌干细胞的自我更新能力，抗体实验为靶向治疗提供依据。
3. **标题**：*HMGB2 mediates DNA damage repair in glioblastoma through interaction with PARP1*
**作者**：Smith J, et al.
**摘要**：利用HMGB2抗体进行免疫共沉淀(Co-IP)和质谱分析，揭示HMGB2与PARP1结合促进胶质母细胞瘤DNA损伤修复，靶向HMGB2可增强放疗敏感性。
4. **标题**：*Developmental regulation of HMGB2 in mouse spermatogenesis*
**作者**：Tanaka K, et al.
**摘要**：通过HMGB2抗体的免疫荧光染色，发现其在精子发生过程中动态表达，调控减数分裂染色体稳定性，缺失导致雄性不育。
(注：以上为模拟内容，实际文献需检索PubMed、Google Scholar等平台，以“HMGB2 antibody”及研究主题为关键词。)
The High Mobility Group Box 2 (HMGB2) protein is a non-histone chromatin-associated protein belonging to the HMGB family, which regulates DNA structure, transcription, replication, and repair. It contains two HMG DNA-binding domains and an acidic tail, enabling interactions with DNA and other proteins to modulate chromatin dynamics. HMGB2 is highly expressed during embryogenesis and in stem/progenitor cells but shows restricted expression in adult tissues, playing roles in cellular differentiation, proliferation, and apoptosis.
HMGB2 antibodies are critical tools for investigating its biological functions and disease associations. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect HMGB2 expression, localization, and DNA-binding activity. Research has linked HMGB2 overexpression to cancer progression (e.g., glioblastoma, colorectal, and ovarian cancers), where it promotes tumorigenesis, metastasis, and chemoresistance. It also participates in inflammatory responses and autoimmune disorders by facilitating cytokine release.
Validating HMGB2 antibody specificity is essential due to homology with HMGB1 and HMGB3. Commercial antibodies are typically raised against unique epitopes within distinct domains. Recent studies also explore HMGB2's role in aging, stem cell regulation, and regenerative medicine, highlighting its dual context-dependent roles in tissue repair and pathological processes. Reliable HMGB2 antibodies thus underpin mechanistic studies and potential therapeutic targeting.
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